| 声门上型喉癌中端粒酶活性的检测及其意义 |
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| 引用本文： | 魏宏权,郭星,付伟能,孙开来. 声门上型喉癌中端粒酶活性的检测及其意义[J]. 临床耳鼻咽喉头颈外科杂志, 1999, 13(9): 387-389 |
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| 作者姓名： | 魏宏权 郭星 付伟能 孙开来 |
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| 作者单位： | 1. 中国医科大学附属第一医院耳鼻咽喉科,沈阳,110001
2. 中国医科大学医学遗传学教研室 |
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| 摘 要： | 目的：探讨端粒酶活性在声门上型喉癌中的表达及其意义。方法：采用端粒重复序列扩增法检测了ＨＥＰ－２喉癌细胞系，２６例声门上型喉癌组织和１５例癌旁组织。结果：ＨＥＰ－２喉癌细胞系呈端粒酶阳性，癌组织的端粒酶阳性率为８４．６％，明显高于癌旁组织的阳性率４０％（Ｐ〈０．０１），喉癌组织端粒酶活性的表达与临床分期和有无颈淋巴结转移未出现出相关性（Ｐ〉０．０５）。结论：端粒酶活化是喉癌发生的重要遗传学改变，但
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| 关 键 词： | 喉肿瘤 端粒酶 端粒重复序列扩增法 |
The detection and clinical significance of telomerase activityin supraglottic carcinomas |
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| H Wei,X Guo,W Fu,K Sun. The detection and clinical significance of telomerase activityin supraglottic carcinomas[J]. Journal of clinical otorhinolaryngology, head, and neck surgery, 1999, 13(9): 387-389 |
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| Authors: | H Wei X Guo W Fu K Sun |
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| Affiliation: | Department of Otorhinolaryngology, the First Affiliated Hospital, China Medical University, Shenyang 110001. |
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| Abstract: | OBJECTIVE: To study the expression and significance of telomerase activity in laryngeal carcinoma. METHOD: We detected telomerase activity of HEP-2 cell line, 26 cases of supraglottic carcinoma tissues and 15 cases of peri-carcinoma tissues with PCR-based assay designated TRAP (for telomeric repeat amplification protocol). RESULT: The telomerase positive rate of supraglottic carcinoma 84.6% (22/26) was obvious higher than that of peri-carcinoma 40% (6/15), P < 0.01. No significant differences in positive rates were found among different groups divided according to clinical stage or metastasis of cervical lymph node, P > 0.05. CONCLUSION: Telomerase activation is related to immortalization process, though not much helpful in diagnosis of supraglottic carcinoma. |
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| Keywords: | Laryngeal neoplasm Telomerase PCR based assay designated for telomeric repeat amplification protocol |
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