| 人重组白细胞介素-8的快速纯化 |
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| 引用本文： | 李卫党,周宝宏,犹春辉,白惠卿,马大龙. 人重组白细胞介素-8的快速纯化[J]. 江苏大学学报(医学版), 1997, 0(2) |
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| 作者姓名： | 李卫党 周宝宏 犹春辉 白惠卿 马大龙 |
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| 作者单位： | 北京医科大学分子免疫室，北京医科大学分子免疫室，北京医科大学分子免疫室，北京医科大学分子免疫室 |
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| 摘 要： | 报道一种快速人重组IL-8纯化方法，大肠杆菌表达的IL-8包涵体经尿素变性后，直接用DEAE柱和SephadexG50柱纯化。纯化的hIL-8经FPLC鉴定，纯度达90%以上。该法为实验室快速、简便纯hIL-8提供了新的技术路线。
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| 关 键 词： | 人重组白细胞介素-8 DEAE Senhadex G50 FPLC |
Fast Purification of Human Recombinant Interleukin-8 |
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| Li Wei Dang, et al. Fast Purification of Human Recombinant Interleukin-8[J]. Journal of Jiangsu University Medicine Edition, 1997, 0(2) |
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| Authors: | Li Wei Dang et al |
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| Affiliation: | Dept of Immunology |
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| Abstract: | The paper reports a test purification method of human recombiant interleukin-8 (hIL-8). hIL-8was purified by denaturation of urea and DEAE column and Sephadwx G50 column. Dialysis was notnpcessary for purification. The purity of purified hIL-8 identified by FPLC was more than 90%. Thisnlethod has characters of simplicity, fastness and higher purity. |
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| Keywords: | Human recombinant interleukin-8 DEAE Sephadex G5O FPLC |
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