丹参多酚酸盐对大鼠急性坏死性胰腺炎心肌损伤的防治作用

目的:探讨丹参多酚酸盐静脉注射对急性坏死性胰腺炎(ANP)大鼠并发心肌损伤的防治作用。方法:42只健康雄性SD大鼠随机均分为对照组、模型组、治疗组,用腹腔注射左旋精氨酸(L-Arg)溶液造模,治疗组大鼠于造模后10 min尾静脉注射丹参多酚酸盐注射液(100 mg/kg)。48 h后处死动物,检测各组血清淀粉酶、心肌组织磷脂酶A2(PLA2)、血清肌钙蛋白I(CTnI)水平,并行心肌组织病理学检查。结果:与对照组比较,模型组与治疗组血清淀粉酶活性、CTnI水平和心肌组织PLA2均明显升高(均P<0.05),但治疗组以上指标均低于模型组(均P<0.05);对照组心肌无病理改变,模型组心肌组织可见灶性炎性细胞浸润、心肌断裂、出血,治疗组心肌间质可见少量炎性浸润。结论:丹参多酚酸盐注射液对大鼠ANP及其并发心肌损伤具有防治作用。

Protective effect of salvianolate on myocardial injury secondary to acute necrotizing pancreatitis in rats

Objective: To investigate the protective effect of salvianolate intravenous injection on the myocardial impairment secondary to acute necrotizing pancreatitis(ANP) in rats. Methods: Forty-two healthy male SD rats were equally randomized into the control group,ANP model group and salvianolate treatment group.The ANP model was induced by intraperitoneal injection of 20% L-Arginine solution and rats of the salvianolate treatment group were given salvianolate(100 mg/kg) injection through tail veil 10 min after ANP induction.All rats were killed 48 h after model establishment,and the serum amylase activity,contents of cardiac troponin I,and phospholipase A2 in myocardial tissues were detected.The pathological examination of the myocardial tissues was performed. Results: Compared with the control group,the activated serum amylase,and serum contents of cardiac troponin I and myocardial phospholipase A2 levels of both the ANP model group and salvianolate treatment group were significantly increased(all P<0.05),but these parameters of the salvianolate treatment group were all lower than those of the ANP model group(all P<0.05).No pathological change was noted in myocardial tissues of the control group,while the myocardial tissues of the ANP model group showed focal inflammatory infiltration with occasional myocyte rupture and hemorrhage,and the salvianolate treatment group exhibited slight inflammatory infiltration in interstitial tissue of myocardium. Conclusion: Salvianolate injection has protective effect on myocardial injury secondary to ANP.