cPLA2和COX-2在肺癌组织中的表达及生物学意义

目的探讨磷脂酶A2（cPLA2）和环氧合酶(COX-2)在肺癌中的表达及生物学意义，为肺癌的靶向治疗提供参考依据。方法采用RT-PCR检测肺癌及癌旁组织中cPLA2、COX-2 mRNA的表达，同时采用免疫组织化学SP法检测相应标本中cPLA2和COX-2蛋白的表达。结果肺癌组织中cPLA2和COX-2 mRNA的表达量均明显高于癌旁组织（P<0.05）。cPLA2蛋白在肺癌组织中的阳性表达率为76.7%（46/60），癌旁组织中未见表达。肺癌组织中cPLA2蛋白的阳性表达率与肿瘤大小、组织类型、TNM分期、分化程度和淋巴结转移均无关（P>0.05）。COX-2蛋白在肺癌组织中的阳性表达率为73.3%（44/60），明显高于在相应癌旁组织中的表达［13.3%（8/60）］（χ² =21.99，P<0.01）。肺癌组织中COX-2蛋白的阳性表达率与肿瘤大小、组织类型无关（P>0.05）；而与淋巴结转移、TNM分期、分化程度有关，随着淋巴结的转移、组织分化程度的降低和临床分期的增加，肺癌组织中COX-2蛋白的阳性表达率逐渐增高，差异有统计学意义（P<0.05）。结论 COX-2和cPLA2的高表达可能在肺癌的发生发展起着重要作用，它们可能为肺癌的早期诊断和开发肺癌的靶向治疗提供一定的临床依据。

Expression and Biological Significance of cPLA2 and COX-2 in Lung Cancer Tissues

Objective To explore the expression and biological significance of cPLA2 and Cox-2 in lung cancer tissues.Methods The expression of cPLA2 and COX-2 in mRNA level in cancerous tissue and tumor-adjacent tissue were detected by RT-PCR,while the immunohistochemical SP method was used to evaluate the expression of cPLA2 and COX-2 proteins in the corresponding tissues.Results The mRNA level of COX-2 and cPLA2 significantly increased(P<0.05)in lung cancer group as compared with those in the tumor-adjacent tissue.The positive expression rate of cPLA2 in cancerous tissue was 76.7%(46/60),but no expression was observed in tumor-adjacent tissue.The positive expression rate of cPLA2 in lung cancer was independent of clinic pathological parameters including size of tumor,histological type,TNM staging,histological grade and lymph node metastasis(P<0.05).The positive expression rate of COX-2 protein in cancerous tissue and tumor-adjacent tissue were 73.3%(44/60)and 13.3%(8/60)(P<0.01).The positive expression rate of cox-2 protein in lung cancer was independent of size of tumor and histological type(P>0.05),but correlated with histological grade,lymph node metastasis and TNM staging.The positive expression rate of COX-2 protein increased gradually with lymph node metastasis,the decrease of histological grade and the increase of TNM staging.There was a significant difference(P<0.05).Conclusion It suggested that the expression of cPLA2 and COX-2 in lung cancer may play an important role in the process of carcinogenesis,and might provide a clinical basis for the early diagnosis and targeted therapy of lung cancer.