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Immunocytochemical labelling of horizontal cells in mammalian retina using antibodies against calcium-binding proteins
Authors:J R?hrenbeck  H W?ssle  C W Heizmann
Institution:1. Max-Planck-Institut für Hirnforschung, Frankfurt F.R.G.;2. Institut für Pharmakologie und Biochemie der Universität Zürich, Zürich Switzerland;1. University of Pennsylvania/Ophthalmology, Philadelphia, PA, USA;2. Center for Advanced Retinal and Ocular Therapeutics, F. M. Kirby Center for Molecular Ophthalmology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA;1. Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, GA, USA;2. James and Jean Culver Vision Discovery Institute, Augusta University, Augusta, GA, USA;3. Department of Physiology, Medical College of Georgia, Augusta University, Augusta, GA, USA;4. Department of Ophthalmology, Medical College of Georgia, Augusta University, Augusta, GA, USA;1. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México D.F., Mexico;2. Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN, México D.F., Mexico;1. Section of Ophthalmology, Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA;2. Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania 19104, USA;1. Institut National de la Santé et de la Recherche Médicale, U1051, Institute for Neurosciences of Montpellier, Montpellier, France;2. University of Montpellier 1, Montpellier, France;3. University of Montpellier 2, Montpellier, France;4. CHRU, Genetics of Sensory Diseases, Montpellier, France;5. Clinical Investigation Centre, University Hospital of Montpellier, Montpellier, France;6. The Marie Curie Laboratory for Membrane Proteins, National University of Ireland, Maynooth, Ireland;7. CHRU Lille, Laboratoire de Biochimie et Biologie Moléculaire, UF Génopathies, Lille, France;8. Laboratoire Neurosciences Fonctionnelles et Pathologies, CNRS FRE 2726, Hôpital Roger Salengro, Lille, France;9. Université Lille Nord de France, Lille, France;10. Institut National de la Santé et de la Recherche Médicale, U968, Paris, France;11. Centre National de la Recherche Scientifique, UMR 7210, Paris, France;12. Sorbonne Universités, Université Pierre et Marie Curie, Paris 06, UMR S968, Paris, France;13. Centre Hospitalier National d''Ophtalmologie des Quinze-Vingts, INSERM, Direction de l''Hospitalisation et de l''Organisation des Soins, Centre d''Investigation Clinique 1423, Paris, France;14. Department of Genetics, INSERM U781, Hôpital Necker Enfants Malades, Université Paris-Descartes, Paris, France;15. Groupe Hospitalier Necker–Enfants Malades, Université Paris-Descartes, Paris, France;p. Paris Descartes University, AP-HP Hôtel-Dieu Hospital, Department of Ophthalmology, Paris, France;q. Clinique Ophtalmologique Sourdille, Nantes, France;r. CHU-Hotel Dieu, Service d''Ophtalmologie, Nantes, France;s. Centre Hospitalier Universitaire de Rennes, Rennes, France;t. Hôpitaux Universitaires de Strasbourg (HUS), Centre de référence pour les affections rares en génétique ophtalmologique (CARGO), Strasbourg, France;u. Centre de génétique, hôpital d''Enfants, université de Dijon, Dijon, France;v. INSERM UMR_S 910, University of Aix-Marseille, Marseille, France;1. Department of Interdisciplinary Program in Biomedical Science, Soonchunhyang Graduate School, Bucheon Hospital, Bucheon, South Korea;2. Laboratory for Translational Research on Retinal and Macular Degeneration, Soonchunhyang University Hospital Bucheon, Bucheon, South Korea;3. Department of Ophthalmology, Soonchunhyang University Hospital Bucheon, Bucheon, South Korea;4. CMLAB, Convergence Technologies for Bio-Medical Science, Seoul, South Korea;5. Department of Ophthalmology, College of Medicine, Soonchunhyang University, Cheonan, Choongchungnam-do, South Korea;6. Ex Lumina Therapeutics and Technologies, Bucheon, South Korea
Abstract:Using antibodies against calcium-binding proteins immunocytochemistry revealed quantitative staining of horizontal cells in whole mount preparations. In cat both A- and B-type horizontal cells, and in monkey probable H1- and H2-horizontal cells were labelled with antibodies against parvalbumin. In rabbit and ox two types of horizontal cell were labelled with antibodies against calcium-binding protein (CaBP-28K). In ox retina processes of horizontal cells were observed descending into the inner plexiform layer (IPL).
Keywords:
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