原子力显微镜对华蟾素作用的SGC-7901细胞的凋亡研究

目的应用原子力显微镜（AFM）研究华蟾素对胃癌SGC-7901细胞的作用，在单细胞水平上分析胃癌SGC-7901细胞超微机构的变化，为进一步探讨华蟾素对胃癌SGC-7901细胞的作用机制奠定基础。方法利用原子力显微镜高分辨率、高灵敏度的特点，观察不同浓度（0、6.25、12.5、25、37.5、50ug/ml）华蟾素对胃癌细胞的作用，以及同等浓度药物作用下，随着作用时间（24h,48h,72h,96h）的延长，观察细胞超微结构的变化。同时，利用流式细胞仪对凋亡情况进行分析。结果药物作用后，细胞开始塌陷变矮。细胞表面结构的几何参数，包括高低差（Rp-v）、均方根粗糙度（Rq）、平均粗糙度（Ra）、平均高度（Meant Ht）均出现明显变化，且加药后的细胞凋亡率明显高于对照组（5.04±1.11）%。结论通过AFM观察细胞表面超微结构的变化，从纳米尺度上研究了华蟾素对胃癌细胞的作用，从另一层面增加了对胃癌细胞的认识，从而使AFM有望成为临床肿瘤诊断的一种新仪器。

Analysis of Gastric Cancer Cell Line SGC-7901 Influenced by Cinobufacini Using AFM

ObjectiveTo characterize the morphology of gastric cancer cell line SGC-7901 influenced by Cinobufacini,which is one of the traditional Chinese medicine reagents. The ultrastructure changes of gastric cancer cell line SGC-7901 interacted by Cinobufacini were analyzed by atomic force microscope (AFM) at single-cell level. MethodThe ultrastructure changes of gastric cancer cell line SGC-7901 interacted by Cinobfacini were analyzed by atomic force microscope (AFM) at single- cell level. And cell apoptotic rate was determined by flow cytometry. ResultCompared to the untreated cell,the size of cells decreased,and the nucleolus crimpled. The cellular membrane ultrastructure,Rp-v,Rq,and Ra value were significantly changed. And flow cytometry data indicated that cell apoptosis rate increased with increasing concentration of Cinobufacini. ConclusionAFM- based experiments which helped us increase the awareness of gastric cancer cell line SGC-7901 at visualization will contribute to shedding new light into the structure-function relationships of cell surfaces at single cell or nanometer level. In other words,AFM could be a new useful tool to diagnose cancer at single cell level.