IL-2和(或)IL-3重组腺病毒直接体内注射对大剂量化疗后小鼠造血功能恢复的影响

目的：检测应用直接腹腔注射白细胞介素２（ＩＬ－２）重组腺病毒和（或）白细胞介素３（ＩＬ－３）重组腺病毒方法对化疗后小鼠造血功能恢复的影响。方法：正常小鼠腹腔注射大剂量环磷酰胺（２００ｍｇ／ｋｇ）２４小时后，直接腹腔注射ＩＬ－２重组腺病毒（Ａｄ－ＩＬ－２）和（或）ＩＬ－３重组腺病毒（Ａｄ－ＩＬ－３），用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）检测相应细胞内细胞因子ｍＲＮＡ，并持续观察小鼠外周血白细胞、股骨单个核细胞、骨髓ＣＦＵ－ＧＭ的恢复情况。结果：腹腔注射Ａｄ－ＩＬ－２和（或）Ａｄ－ＩＬ－３组小鼠腹腔细胞可测及相应细胞因子ｍＲＮＡ，血清中可测及相应细胞因子；腹腔注射Ａｄ－ＩＬ－３组小鼠外周血白细胞、股骨单个核细胞、骨髓ＣＦＵ－ＧＭ最低值均显著提高（Ｐ＜０．０５），但腹腔注射Ａｄ－ＩＬ－２组小鼠对化疗后小鼠造血功能无明显影响，联合应用Ａｄ－ＩＬ－２和Ａｄ－ＩＬ－３与单独应用Ａｄ－ＩＬ－３相比无显著性差异。结论：腹腔直接注射Ａｄ－ＩＬ－３能促进化疗后小鼠造血功能的恢复。

The effects of intraperitoneal injection of IL-2 recombinant adenovirus and/or IL-3 recombinant adenovirus on the hematological recovery of murine model receiving high dose chemotherapy

OBJECTIVE: To investigate the effect of Ad-IL-2 and/or Ad-IL-3 on the hematological recovery of murine model receiving high dose chemotherapy. METHODS: Ad-IL-2 and/or Ad-IL-3 were i.p. injected into experimental mice after high dose cyclophosphamide(200 mg/kg). RT-PCR analysis of peritoneal cells and cytokine levels in murine serum were detected to confirm gene transfering. The number of peripheral WBC, femur MNC and CFU-GM in bone marrow were observed continuously. RESULTS: After i.p. injection of Ad-IL-2 and/or Ad-IL-3, the transfected IL-2 and IL-3 gene were identified by RT-PCR analysis of peritoneal cells, high level of IL-2 and/or IL-3 were detected in murine serum. After i.p. injection of AD-IL-3, the number of WBC, femur MNC and CFU-GM in bone marrow significantly increased, while i.p. injection of Ad-IL-2 had no effect on improving hematological recovery. And the effect of i.p. injection of both Ad-IL-2 and Ad-IL-3 was not significantly different from that of injection either of them. CONCLUSION: i.p. injection of Ad-IL-3 can increase hematological recovery after high dose chemotherapy.