Simple coating with fibronectin fragment enhances stainless steel screw osseointegration in healthy and osteoporotic rats |
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| Institution: | 1. Orthopaedic Research & Biotechnology Unit, The Children''s Hospital at Westmead, Sydney, Australia;2. Discipline of Paediatrics and Child Health, Sydney Medical School, University of Sydney, Sydney, Australia;3. Novartis Pharma, Basel, Switzerland;1. Department of Prosthodontics, Ninth People''s Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China;2. Oral Bioengineering and Regenerative Medicine Lab, Shanghai Key Laboratory of Stomatology, Ninth People''s Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China;3. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China;4. Shanghai Institute of Digestive Surgery and Department of Surgery, Rui Jin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China;5. Department of Oral and Maxillofacial Surgery, Ninth People''s Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China |
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| Abstract: | Metal implants are widely used to provide structural support and stability in current surgical treatments for bone fractures, spinal fusions, and joint arthroplasties as well as craniofacial and dental applications. Early implant-bone mechanical fixation is an important requirement for the successful performance of such implants. However, adequate osseointegration has been difficult to achieve especially in challenging disease states like osteoporosis due to reduced bone mass and strength. Here, we present a simple coating strategy based on passive adsorption of FN7-10, a recombinant fragment of human fibronectin encompassing the major cell adhesive, integrin-binding site, onto 316-grade stainless steel (SS). FN7-10 coating on SS surfaces promoted α5β1 integrin-dependent adhesion and osteogenic differentiation of human mesenchymal stem cells. FN7-10-coated SS screws increased bone-implant mechanical fixation compared to uncoated screws by 30% and 45% at 1 and 3 months, respectively, in healthy rats. Importantly, FN7-10 coating significantly enhanced bone-screw fixation by 57% and 32% at 1 and 3 months, respectively, and bone-implant ingrowth by 30% at 3 months compared to uncoated screws in osteoporotic rats. These coatings are easy to apply intra-operatively, even to implants with complex geometries and structures, facilitating the potential for rapid translation to clinical settings. |
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| Keywords: | Integrin Cell adhesion Bone Implant |
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