Tissue-engineered acellular small diameter long-bypass grafts with neointima-inducing activity |
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| Affiliation: | 1. Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Fujishiro-dai, Suita, Osaka 565-8565, Japan;2. Department of Biobased Materials Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan;3. Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka 565-8680, Japan;4. Department of Biomedical Engineering, Osaka Institute of Technology, 5-16-1, Omiya, Asahi-ku, Osaka 535-8585, Japan;1. Key Laboratory of Textile Science and Technology of Ministry of Education and College of Textiles, Donghua University, 2999 North Renmin Road, Shanghai 201620, China;2. Department of Engineering, University of Cambridge, Cambridge CB2 1PZ, UK;3. Department of Surgery, Laval University and Axe of Regenerative Medicine, Research Center CHU, Quebec, Canada;1. State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China;2. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China;3. Institute of Biomedical Engineering, Chinese Academy of Medical Science, Tianjin 300192, China;4. Department of Biochemistry & Molecular Biology, Libin Cardiovascular Institute of Alberta, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada T2N 4N1;1. Institute for Regenerative Medicine (IREM), Center for Therapy Development and Good Manufacturing Practice, University of Zurich, Zurich, Switzerland;2. Division of Trauma Surgery, Center for Clinical Research, University Hospital Zurich, University of Zurich, Zurich, Switzerland;3. Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich, Zurich, Switzerland;4. Wyss Zurich, University of Zurich and ETH Zurich, Zurich, Switzerland;5. Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland |
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| Abstract: | Researchers have attempted to develop efficient antithrombogenic surfaces, and yet small-caliber artificial vascular grafts are still unavailable. Here, we demonstrate the excellent patency of tissue-engineered small-caliber long-bypass grafts measuring 20–30 cm in length and having a 2-mm inner diameter. The inner surface of an acellular ostrich carotid artery was modified with a novel heterobifunctional peptide composed of a collagen-binding region and the integrin α4β1 ligand, REDV. Six grafts were transplanted in the femoral–femoral artery crossover bypass method. Animals were observed for 20 days and received no anticoagulant medication. No thrombogenesis was observed on the luminal surface and five cases were patent. In contrast, all unmodified grafts became occluded, and severe thrombosis was observed. The vascular grafts reported here are the first successful demonstrations of short-term patency at clinically applicable sizes. |
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| Keywords: | Small-diameter vascular graft Long bypass Acellular Tissue engineering Neointima |
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