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A plug-and-play ratiometric pH-sensing nanoprobe for high-throughput investigation of endosomal escape
Affiliation:1. Chemical Engineering, Koch Institute for Integrative Cancer Research, Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, USA;2. Aramco Research Center, Boston, MA, USA;1. Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA, USA;2. Instituto de Investigaciones en Materiales, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico;3. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA;4. Facultad de Odontologia, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico;1. Quantum Beam Science Center, Japan Atomic Energy Agency, Tokai, Ibaraki 319-1195, Japan;2. Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan;3. Graduate School of Science, University of Hyogo, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan;4. Neutron Science Section, J-PARC Center, Tokai, Ibaraki 319-1195, Japan;1. Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA;2. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53705, USA;3. Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53705, USA;4. Department of Surgery, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705, USA;1. Department of Chemistry, University of New Orleans, New Orleans, LA 70148, USA;2. Southern Regional Research Center, New Orleans, LA 70124, USA
Abstract:An important aspect in the design of nanomaterials for delivery is an understanding of its uptake and ultimate release to the cytosol of target cells. Real-time chemical sensing using a nanoparticle-based platform affords exquisite insight into the trafficking of materials and their cargo into cells. This versatile and tunable technology provides a powerful tool to probe the mechanism of cellular entry and cytosolic delivery of a variety of materials, allowing for a simple and convenient means to screen materials towards efficient delivery of therapeutics such as nucleic acids.
Keywords:Nanoparticle  Endosomal escape  Chemical sensing  Delivery  Nanotechnology
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