Functional remodeling after vocal fold injury by small intestinal submucosa gel containing hepatocyte growth factor |
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| Affiliation: | 1. Department of Otorhinolaryngology-Head and Neck Surgery, Republic of Korea;2. Translational Research Center, Inha University School of Medicine, Incheon, Republic of Korea;3. Department of Molecular Science and Technology, Ajou University, Suwon, Republic of Korea;1. Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan;2. Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 300, Taiwan;1. Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, China;2. Department of General Surgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China;3. Key Laboratory of Smart Drug Delivery, Ministry of Education & PLA, School of Pharmacy, Fudan University, Shanghai 201203, China;1. Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, and The Affiliated Hospital of Stomatology of Chongqing Medical University, Chongqing, China;2. Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA;3. Department of Cell Biology, Third Military Medical University, Chongqing 400038, China;4. Ministry of Education Key Laboratory of Diagnostic Medicine, and The Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China;5. Department of Surgery, The Affiliated Zhongnan Hospital of Wuhan University, Wuhan, China;6. School of Bioengineering, Chongqing University, Chongqing, China;1. Universidad Nacional de Colombia, Bogotá, Colombia;2. Universidad Santo Tomás, Bogotá, Colombia;1. Interdisciplinary Program of Integrated Biotechnology, Sogang University, 35 Baekbeom-ro, Mapo-Gu, Seoul 121-742, Republic of Korea;2. Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, Republic of Korea;3. Chemistry Department, Faculty of Science, Assiut University, Assiut 71516, Egypt |
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| Abstract: | A biomaterial derived from porcine small intestinal submucosa (SIS) was used in smart drug delivery and tissue remodeling. SIS suspensions were easily formulated by simple mixing with the drug of choice and formed an in situ gel upon injection into tissues, enabling them to act as protein drug depots. This study was conducted to determine whether functional remodeling of an injured vocal fold (VF) could be achieved by hepatocyte growth factor (HGF)-containing SIS in situ-forming gel after VF injury in a rabbit model. To accomplish this, we loaded HGF in SIS suspensions and observed a gradual, sustained release of HGF for at least 21 days in vitro. Evaluation of the in vivo efficacy demonstrated that the HGF and HGF-loaded SIS treated VFs showed improved mucosal healing when compared with the PBS-injected VFs. Histopathological evaluations revealed that treatment with the HGF/SIS group alone successfully ameliorated the deposition of type I collagen and increased synthesis of hyaluronic acids relative to the PBS group at three months post-injury. Functional analyses showed that the HGF/SIS group prevented deterioration of mucosal vibration and induced significant improvement in the mean viscoelastic modulus, but that other groups failed to achieve functional rescue of VF biomechanics. Additionally, the VF oscillation in the HGF/SIS group was superior to that in the HGF group. The results of this study suggest that SIS in situ gel has the potential for use as an HGF delivery carrier for enhancement of wound healing and improvement of functional remodeling following VF injury. |
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| Keywords: | Hepatocyte growth factor Vocal folds Wound healing Extracellular matrix Biomimetic materials |
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