Efficient differentiation of stem cells encapsulated in a cytocompatible phospholipid polymer hydrogel with tunable physical properties |
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| Institution: | 1. Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;2. Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;1. G.G. Devyatykh Institute of Chemistry of High-Purity Substances RAS, Nizhny Novgorod, Russia;2. N.I. Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia;3. Institute of Applied Physics RAS, Nizhny Novgorod, Russia;1. Key Laboratory of Green Catalysis of Higher Education Institutes of Sichuan, College of Chemistry and Pharmaceutical Engineering, Sichuan University of Science and Engineering, Zigong 643000, PR China;2. College of Materials and Chemical Engineering, Sichuan University of Science and Engineering, Zigong 643000, PR China;1. Bio-Nanotechnology Laboratory, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulatur, Kancheepuram Dist., Chennai 603203, India;2. Biochemical Sciences Division, National Chemical Laboratory, Pashan Road, Pune 411008, India;3. Department of Biotechnology, School of Life Sciences, Pondicherry University, Kalapet, Pondicherry 605014, India;1. Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan;2. Project Facilitation Department, Sohyaku. Innovative Research Division, Mitsubishi Tanabe Pharma Corporation, Aoba-ku, Yokohama-shi, Kanagawa 227-0033, Japan;1. National Institute of Nursing Research, National Institutes of Health, Bethesda, Maryland, USA;2. Vassar College, Poughkeepsie, New York, USA |
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| Abstract: | A large number of lineage-committed progenitor cells are required for advanced regenerative medicine based on cell engineering. Due to their ability to differentiate into multiple cells lines, multipotent stem cells have emerged as a vital source for generating transplantable cells for use in regenerative medicine. Increment in differentiation efficiency of the mesenchymal stem cell was obtained by using hydrogel to adjust the proliferation cycle of encapsulated cells to signal sensitive phase. Three dimensional (3-D) polymer networks composed of poly(2-methacyloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-p-vinylphenylboronic acid (VPBA)) (PMBV) and poly(vinyl alcohol) (PVA) were prepared as a hydrogel. The proliferation of cells encapsulated in the PMBV/PVA hydrogel was highly sensitive to the storage modulus (G′) of the hydrogel. That is, when the G′ value of the hydrogel was higher than 1.0 kPa, the cell proliferation was ceased and the proliferation cycle of cells was converged to G1 phase, whereas when the G′ value was below 1.0 kPa, cell proliferation proceeded. By changing the G′ value of hydrogels under encapsulation the cells, proliferation cycle of encapsulated mesenchymal stem cells was regulated to G1 phase and thus signal sensitivity were increased. 3-D polymer networks as hydrogels with tunable physical properties can be effectively used to control proliferation and lineage-restricted differentiation of stem cells. |
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| Keywords: | Mesenchymal stem cell Cell cycle Differentiation Phospholipid polymer hydrogel |
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