Mitigation of hypertrophic scar contraction via an elastomeric biodegradable scaffold |
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| Affiliation: | 1. Department of Biomedical Engineering, Duke University, Box 90281, Durham, NC 27708, USA;2. Division of Plastic and Reconstructive Surgery, Department of Surgery, Duke University Medical Center, Box 3181, Durham, NC 27710, USA;3. Biomedical Research Institute, Center for Biomaterials, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea;4. Department of Pathology, Duke University Medical Center, Box 3712, Durham, NC 27710, USA;5. Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA;1. Department of Materials and Textiles, Oriental Institute of Technology, Pan-Chiao, 22064, Taiwan, ROC;2. Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 10607, Taiwan, ROC;3. R&D Center for Membrane Technology, Chung Yuan University, Jongli, Taoyuan, 32023, Taiwan, ROC;4. Department of Chemical and Materials Engineering, Vanung University, Jongli, Taoyuan, 32091, Taiwan, ROC;5. Department of Textile Engineering, Chinese Culture University, Taipei, 11114, Taiwan, ROC;6. Graduate School of Materials Applied Technology, Taoyuan Innovation Institute of Technology, Jongli, Taoyuan, 32091, Taiwan, ROC;1. McGowan Institute for Regenerative Medicine, University of Pittsburgh, USA;2. Dept. of Surgery, University of Pittsburgh, USA;3. Dept. of Bioengineering, University of Pittsburgh, USA;4. Stem Cell Research Center, University of Pittsburgh, USA;5. Institute for Complex Engineered Systems, Carnegie Mellon University, USA;6. Thomas E. Starzl Transplantation Institute, University of Pittsburgh, USA;7. Dept. of Orthopedic Surgery, University of Pittsburgh, USA;8. Dept. of Chemical Engineering, University of Pittsburgh, Pittsburgh, PA, 15219, USA;1. Department of Biomedical Sciences for Health (SCIBIS), University of Milan, Via G. Colombo 71, 20133 Milan, Italy;2. Center of Sport Medicine, Don Gnocchi Foundation, Via Capecelatro 66, 20148 Milan, Italy;1. Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, South Korea;2. Division of Bioengineering, Incheon National University, Incheon 406-772, South Korea |
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| Abstract: | Hypertrophic scar (HSc) occurs in 40–70% of patients treated for third degree burn injuries. Current burn therapies rely upon the use of bioengineered skin equivalents (BSEs), which assist in wound healing but do not prevent HSc contraction. HSc contraction leads to formation of a fixed, inelastic skin deformity. We propose that BSEs should maintain their architecture in the wound bed throughout the remodeling phase of repair to prevent HSc contraction. In this work we study a degradable, elastomeric, randomly oriented, electrospun micro-fibrous scaffold fabricated from the copolymer poly(l-lactide-co-ε-caprolactone) (PLCL). PLCL scaffolds displayed appropriate elastomeric and tensile characteristics for implantation beneath a human skin graft. In vitro analysis using human dermal fibroblasts demonstrated that PLCL scaffolds decreased myofibroblast formation as compared to an in vitro HSc contraction model. Using a validated immune-competent murine HSc contraction model, we found that HSc contraction was significantly greater in animals treated with standard of care, Integra, as compared to those treated with collagen coated-PLCL (ccPLCL) scaffolds. Finally, wounds treated with ccPLCL were significantly less stiff than control wounds at d30 in vivo. Together, these data suggest that scaffolds which persist throughout the remodeling phase of repair may represent a clinically translatable method to prevent HSc contraction. |
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| Keywords: | Animal model Biodegradation Burn Collagen Mechanical properties Microstructure |
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