检测白血病细胞增殖力的MTS/pms比色分析法的建立

为了探讨建立一种更为快速、安全、灵敏的白血病细胞增殖力的检测方法，本研究应用HL—60和K562细胞可转化MTS／pms形成水溶性待测产物，其光密度值在492nm波长下半自动测定的特点，研究了MTS／pms比色分析法取代MTT和INT用于白血病细胞增殖力的检测。结果表明，只有活的白血病细胞才能转化MTS／pms形成待测产物，且白血病细胞数与所测光密度值(OD)之间具有良好的相关性(γ＝0．963)；与MTT和INT法相比较，MTS／pms比色分析法中所形成的最终待测产物为水溶性，省略了上清波去除和加入有机溶剂的步骤。结论：MTS／pms比色分析法对白血病细胞增殖力的检测更具有快速、安全、准确、灵敏的优点。

Development of MTS/pms Colorimetric Assay in the Proliferation of Leukemic Cells

In order to establish a new more rapid, safe and sensitive colorimetric assay for the proliferation of leukemic cells, MTS/pms has been developed. This automated colorimetric assay is based on the characteristic of viable and metabolically active leukemic cells to cleave MTS/pms into a water-soluble product whose optical density is determined at 492 nm by an automated microtiter-plate reader photometer. The results indicated that only active leukemic cells cleaved MTS/pms into product measured, and dead cells did not reduce MTS/pms. A linear relations hip were found between the viable cell number and optical density of MTS/pms cleaved by HL-60 and K562 cell (r = 0.963). Compared with MTT and INT assays, the reduced product of MTS/pms is water-soluble. It is concluded that MTS/pms colorimetric assay is more rapid, accurate and sensitive for the bioassay of proliferation of leukemic cells.