RP-HPLC法测定灯盏生脉提取物中野黄芩苷的血药浓度

目的测定中药复方制剂灯盏生脉提取物灌胃后大鼠血浆中野黄芩苷的浓度。方法采用液-液萃取法制备血液样品。以芦丁为内标,采用C18柱,乙腈-体积分数为0.05%的磷酸(体积比为65∶320)为流动相,流速为1.0 mL.min-1,检测波长为335 nm,柱温为40℃。结果野黄芩苷血浆中质量浓度在0.05~5.00 mg.L-1(r=0.997 9),定量下限为0.05 mg.L-1,日内、日间精密度均小于15%。在质量浓度为0.1、0.5、4.0 mg.L-1时,提取回收率分别为(81.6±3.6)%、(79.2±3.0)%和(78.7±2.4)%。主要药动学参数为:ke=(0.269±0.035)h-1、t1/2=(2.61±0.36)h、AUC0-24=(25.272±5.682)mg.h.L-1。结论该方法可用于灯盏生脉提取物中野黄芩苷的药动学研究。

Determination of scutellarin from dengzhanshengmai extracts in rat plasma by RP-HPLC

Objective Determination of scutellarin in rat plasma after ig. administration of dengzhanshengmai liquid were investigated. Methods The plasma samples were prepared using a liquied-liquied extraction. Rutin was used as the internal standard. A C₁₈ column was used, with the mobile phase of acetonitrile-0.05%(φ) phosphoric acid (V:V=65:320) at the flow rate of 1.0 mL·min^-1, at the detection wavelength of 335 nm, column temperature of 40 ℃. Results The calibration curve of scutellarin was linear in the concentration range of 0.05-5.00 mg·L^-1 (r= 0.997 9). The lower limit of quantification was 0.05 mg·L^-1.The RSDs of inter-and intra-day were less than 15%. The average extraction recoveries were (81.6±3.0)%, (79.2±2.4)% and (78.7±1.9)% at concentrations of 0.1, 0.5 and 4.0 mg·L^-1 , respectively. The main pharmacokinetic parameters: k_e= (0.275±0.036) h^-1, t_1/2= (2.57±0.36) h, AUC_0-24= (25.257±5.190) mg?h?L^-1. Conclusions This method is available at the study of the pharmacokinetics of scutellarin from dengzhanshengmai liquid.