神经活性物质对小鼠学习记忆能力及耐力的影响

目的：观察神经活性物质（胚脑组织提取物，NAS）对小鼠学习、记忆能力提高的作用，以及对小鼠耐力的影响。方法：用自制的复杂迷宫检测小鼠的迷宫觅食时间及行走的错误次数，测定小鼠负重5％体重游泳至淹死的时间。将实验动物随机分为五组：生理盐水组（A）、脑活素组（B）和三个不同浓度（C：0.1mg、D:0.2mg、E:0.4mg)的NAS治疗组。结果：①A组迷宫觅食时间治疗前后无显著性差异（P＞0.1),B、C、D、E组治疗后迷宫觅食时间缩短，治疗前后有显著性差异或有非常显著性差异（依次为P＜0.005、P＜0.05、P＜0.001、P＜0.001)；②治疗前后小鼠行走的错误次数A组和B组无显著差异性（P＞0.2和P＞0.5)，C、D、E组有显著性差异（依次为P＜0.02、P＜0.02、P＜0.01)；③小鼠负重5％体重游泳，其淹死的时间五组方差分析有显著性差异（P＜0.05)。但组间比较只有C组与E组（P＜0.05)和D组与E组（P＜0.01)有差异。结论：NAS可能提高小鼠的学习、记忆能力，也可增强小鼠的耐力。但其腹腔注射≥20mg/kg组体重时，小鼠的耐力反而下降。

Effect of the Nervous Active Factors on Learning,Memory, and Stamina of Mice

Objective: To study the effect of fetal brain extract (Nerval Active Factors, NAF) on increased learning, memory,and stamina of mice.Methods: The time of maze-looking for food and the frequency of walking road error were tested by using a complicated maze. We test the drowned time by carrying 5% body weight on mice back.The experimental mice were divided into five groups at random: physiological saline group (A),Cerebrolysin group(B),NAS groups(C: 0.1 mg,D: 0.2mg,E:0.4 mg).Results:(1)The amount of time the mice-looked for food was not significantly changed in the mice of group A (P>0.1) and was significantly shorter in groups B,C,D,and E(P<0.005,P<0.05,P<0.001,P<0.001) after treatment.(2)The frequency of walking road error was not significantly changed in groups A and B(P>0.2,P>0.5) and was significantly decreased in groups C,D and E(P<0.02,P<0.02,P<0.01) after treatment.(3)Drowned time of mice carrying 5% body weight on back had significant difference in five groups by analysis of variance(P<0.05) after treat-ment,but this difference derived from C and E groups(P<0.05)and D and E groups (P<0.01).Conclusion:NAS can raise learning,memory,and stamina of mice.However, on the contrary, when the amount of NAF used by i.p was equal to or beyond 20 mg/kg b.w,stamina of mice decreases.