Tissue factor activity in HeLa cells measured with a continuous chromogenic assay and ELISA reader |
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| Authors: | S D Carson P G Archer |
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| Institution: | 1. Department for Advanced Dental Research, Center for Development of Advanced Medicine for Dental and Oral Diseases, Japanese National Center for Geriatrics and Gerontology, Obu, Japan;2. Division of Oral and Dental Surgery, Department of Advanced Medicine, Japanese National Center for Geriatrics and Gerontology, Obu, Japan;3. Department of Geriatric Dentistry, Showa University, School of Dentistry, Tokyo, Japan;1. Department of Breast Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu, 210029, People''s Republic of China;2. Department of Breast Surgery, Baoying Maternal and Child Health Hospital, 120 Anyi East Road, Yangzhou, Jiangsu 225800, People''s Republic of China;3. Department of Thyroid-Breast Surgery, Nanjing Pukou Hospital, The Fourth Affiliated Hospital of Nanjing Medical University, 18 Puyuan Road, Nanjing, Jiangsu 210031, People''s Republic of China;1. P.G. & Research Department of Zoology, The New College, Chennai 600014, Tamil Nadu, India;2. Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia;3. Department of Herbal and Environmental Science, Tamil University, Thanjavur 613 005, Tamil Nadu, India;4. Department of Zoology, Pachaiyappa''s College for Men, Kanchipuram 631 501, Tamil Nadu, India;1. Université de Lyon, France;2. Univ Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, UMR5023, Ecologie des Hydrosystèmes Naturels et Anthropisés, Villeurbanne, F-69622, France;3. Univ Lyon, Université Claude Bernard Lyon 1, VetAgro Sup, CNRS, UMR5557, INRA UMR1418, Ecologie Microbienne, Research Team “Bacterial Opportunistic Pathogens and Environment”, Marcy L''Etoile, F-69280, France;1. University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zátiší 728/II, 389 25, Vodňany, Czech Republic;2. Young Researchers and Elite Club, Lahijan Branch, Islamic Azad University, Lahijan, Iran |
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| Abstract: | Tissue factor activity expressed by Hela cells cultured in 96-well plates has been quantitated in situ using a continuous spectrophotometric assay. Following the assay, cells assayed without physical disruption remained as viable as cells not subjected to the assay. Very little (or no) tissue factor was expressed in nondisrupted cells relative to that available in cells disrupted by freeze-thawing and sonication. Total tissue factor activity (that available in disrupted cells) decreased not as a simple function of time after subculturing, but was inversely related to cell density. |
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