| 自制脱蛋白骨与骨髓基质细胞的生物相容性研究 |
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| 引用本文： | 刘日光,杨述华,尹培荣,尚显文,易诚青. 自制脱蛋白骨与骨髓基质细胞的生物相容性研究[J]. 中华创伤骨科杂志, 2004, 6(11): 1265-1268 |
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| 作者姓名： | 刘日光 杨述华 尹培荣 尚显文 易诚青 |
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| 作者单位： | 1. 550004,贵阳市,贵阳医学院附属医院骨科
2. 430030,武汉市,华中科技大学同济医学院附属同济医院骨科 |
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| 基金项目： | 国家自然科学基金项目(30170945)，贵州省科学技术基金项目(3049) |
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| 摘 要： | 目的 探讨表面脱钙的脱蛋白异体骨与骨髓基质细胞的生物柑容性，为骨组织工程提供合适的支架材料。方法 用经物理与化学方法处理而制得的脱蛋白骨与骨髓基质细胞（MSCs)复合培养，进行形态学观察、细胞增殖、ALP的活性、骨钙素的分泌与蛋白质含量检测；扫描电镜观察细胞在脱蛋白骨材料上的情况。结果 骨髓基质细胞能在脱蛋白骨上贴附、增殖，其生长及功能不受影响。结论 本方法所制的脱蛋白骨与MSCs具有良好的生物相容性，可作为MSCs的载体应用于骨组织工程。
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| 关 键 词： | 脱蛋白骨 组织工程 骨髓基质细胞 |
| 文章编号： | 1671-7600(2004)11-1265-04 |
| 修稿时间： | 2003-11-24 |
Biocompatibility of self- processed deproteinized bone with marrow stromal cells cultured in vitro |
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| LIU Ri guang,YANG Shu hua,YIN Pei rong,SHANG Xian wen,YI Cheng qing. Biocompatibility of self- processed deproteinized bone with marrow stromal cells cultured in vitro[J]. Chinese Journal of Orthopaedic Trauma, 2004, 6(11): 1265-1268 |
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| Authors: | LIU Ri guang YANG Shu hua YIN Pei rong SHANG Xian wen YI Cheng qing |
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| Affiliation: | LIU Ri guang1,YANG Shu hua2,YIN Pei rong1,SHANG Xian wen1,YI Cheng qing21Department of Orthopaedics,Affiliated Hospital of Guiyang Medical College,Guizhou 550004,China2Department of Orthopaedics,Union Hospital,Tongji Medical College,Huazhong Science and Technology University,Wuhan 430022,ChinaSupported by China State Foundation for Natural Sciences |
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| Abstract: | Objective To study the biocompatibility of deproteinized bone processed by our improved methods with marrow stromal cells (MSCs) cultured in vitro. Methods MSCs were cultured in vitro with deproteinized bone processed physically and chemically. The morphological characters, cell proliferation, protein content, ALP and BGP were detected. Results MSCs could be attached to and extended on the deproteinized bone, and they normally grew, proliferated,and had active functions. Conclusion The results show that the self processed deproteinized bone has good biocompatibility with MSCs and can be used as biomaterials for transplantation in tissue engineering. |
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| Keywords: | Deproteinized bone Tissue engineering Marrow stromal cells(MSCs) |
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