Use of a selective enrichment broth to recover Clostridium difficile from stool swabs stored under different conditions |
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Authors: | Arroyo Luis G Rousseau Joyce Willey Barbara M Low Don E Staempfli Henry McGeer Allison Weese J Scott |
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Institution: | Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada. larroyo@uoguelph.ca |
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Abstract: | The recovery of Clostridium difficile from the stools of patients with C. difficile-associated diarrhea was evaluated by use of an enrichment broth (cycloserine-cefoxitin fructose broth supplemented with 0.1% sodium taurocholate TCCFB]) and was compared to that from selective agar (cycloserine-cefoxitin fructose agar CCFA]) and alcohol shock followed by inoculation onto blood agar (AS-BA). TCCFB was superior to CCFA and AS-BA, and neither the storage time nor the storage temperature affected the recovery rate. |
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