| 藕节炭中3-表白桦脂酸含量的测定 |
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| 引用本文： | 刘善新,王萌,靳光乾,郭承军,苏本正.藕节炭中3-表白桦脂酸含量的测定[J].中国中医药信息杂志,2009,16(6):54-55. |
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| 作者姓名： | 刘善新 王萌 靳光乾 郭承军 苏本正 |
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| 作者单位： | 1. 山东省中医药研究院,山东,济南,250014
2. 山东中医药大学,山东,济南,250355 |
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| 摘 要： | 目的建立藕节炭中3-表白桦脂酸含量的测定方法。方法采用高效液相色谱法。色谱柱：LichrospherC18（4．6mm×150mm，5μm）；流动相：乙腈-0．2％冰醋酸（75：25），流速：1mL／min；柱温：30℃；检测波长：207nm。结果3-表白桦脂酸的进样量在0．3654～3．654gg范围内线性关系良好，r=0．9996，平均回收率为98．83％，RSD=2．47％。不同地区市售藕节炭中3-表白桦脂酸的含量最高为0．5758％，最低为0．0483％。结论本法简便、准确、重复性好，可作为藕节炭中3-表白桦脂酸的含量测定方法。
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| 关 键 词： | 藕节炭 3-表白桦脂酸 高效液相色谱法 含量测定 |
Content Determination of 3-Epibetulinic Acid in Nodus Nelumbinis Rhizomatis Charcoal |
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| Institution: | LIU Shan-xin, WANG Meng, JIN Guang-qian, et al (Shandong Academy of Chinese Medicine, Jinan 250014, China) |
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| Abstract: | Objective To establish a method for the content determination of 3-epibetulinic acid in Nodus Nelumbinis Rhizomatis Charcoal. Methods HPLC was used on Lichrospher C18 column （4.6 mm×150 mm, 5 μm） with mobile phase of acetonitrile-0.2% glacial acetic acid （75 ： 25）, the flow rate of 1 mL/min, the column temperature of 30 ℃ and the detection wavelength of 207 nm. Results 3-epibetulinic acid has a good linearity within 0.365 4-3.654 0 μg （r=0.999 6） and the average recovery was 98.83% with RSD= 2.47%. The content of 3-epibetulinic acid in Nodus Nelumbinis Rhizomatis Charcoal from different region varied from 0.048 3% to 0.575 8%. Conclusion This method is simple, accurate, with good reproducibility, and can be used for the content determination of 3-epibetulinic acid in Nodus Nelumbinis Rhizomatis Charcoal. |
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| Keywords: | Nodus Nelumbinis Rhizomatis Charcoal 3-epibetulinic acid HPLC content determination |
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