Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy |
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| Authors: | Jennifer J. Hunter Benjamin Masella Alfredo Dubra Robin Sharma Lu Yin William H. Merigan Grazyna Palczewska Krzysztof Palczewski David R. Williams |
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| Affiliation: | 1.Flaum Eye Institute, University of Rochester, 601 Elmwood Avenue, Rochester, NY, 14642, USA;2.Institute of Optics, University of Rochester, 601 Elmwood Avenue, Rochester, NY, 14642, USA;3.Center for Visual Science, University of Rochester, 601 Elmwood Avenue, Rochester, NY, 14642, USA;4.Polgenix, Inc., 11000 Cedar Avenue, Suite 260, Cleveland, OH, 44106, USA;5.Department of Pharmacology, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106, USA |
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| Abstract: | In vivo two-photon imaging through the pupil of the primate eye has the potential to become a useful tool for functional imaging of the retina. Two-photon excited fluorescence images of the macaque cone mosaic were obtained using a fluorescence adaptive optics scanning laser ophthalmoscope, overcoming the challenges of a low numerical aperture, imperfect optics of the eye, high required light levels, and eye motion. Although the specific fluorophores are as yet unknown, strong in vivo intrinsic fluorescence allowed images of the cone mosaic. Imaging intact ex vivo retina revealed that the strongest two-photon excited fluorescence signal comes from the cone inner segments. The fluorescence response increased following light stimulation, which could provide a functional measure of the effects of light on photoreceptors.OCIS codes: (010.1080) adaptive optics, (330.4460) Ophthalmic optics and devices, (330.5310) Vision – photoreceptors, (330.7327) Visual optics, ophthalmic instrumentation |
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