视网膜缺血再灌注损伤后视网膜神经节细胞pax6的表达变化与意义

【摘要】 目的 探索视网膜缺血再灌注损伤后视网膜神经节细胞pax6的表达变化及意义。 设计 实验研究。研究对象 缺血再灌注损伤大鼠视网膜。方法 成年健康雄性Sprague-Dawley大鼠 30只，随机选取5只作为空白对照组，其余25只为视网膜缺血再灌注损伤组，采用升高右眼眼压的 方法制作视网膜缺血再灌注损伤模型。视网膜缺血再灌注后1、2、4、6、8周分5组，每组5只，不 同时间点取右眼行免疫荧光染色，观察视网膜神经节细胞中pax6表达情况。主要指标 pax6的表达 。结果 视网膜缺血再灌注损伤后随着时间推移视网膜各层逐渐出现pax6表达阳性的细胞，对照组 视网膜神经节细胞pax6表达阳性率为（1.28±1.41）%，损伤后1、2、4、6、8周分别为（0.99± 1.23）%、（14.45±2.72）%、（50.88±4.73）%、（71.00±4.72）%、（78.80±4.62）% （F=1.350，P＜0.0001）。各组与对照组两两比较，缺血后1周视网膜神经节细胞pax6表达阳性率 差异无统计学意义（P=0.835），缺血再灌注损伤2、4、6、8周视网膜神经节细胞pax6表达阳性率 均明显升高（P均＜0.0001）。结论 视网膜缺血再灌注损伤后视网膜各层均出现pax6表达阳性细胞 ，视网膜缺血再灌注损伤能诱导视网膜内源性干细胞激活。（眼科， 2012, 21： 414-417）

Significance of pax6 expression in retinal ganglion cells after ischemia-reperfusion injury in rats

Objective To observe the expression of pax6 in retinal ganglion cells （RGC） and its significance after retinal ischemia-reperfusion （RIR） injury. Design Experimental study. Participants Rat model of RIR injury. Methods 30 adult female SD rats were randomly divided into 6 groups： group l（n=5） normal control; group 2-6 （n=5 for each group） RIR injury groups （post injury 1 week, 2 weeks, 4 weeks, 6 weeks and 8 weeks respectively）. The intraocular pressure （IOP） of right eye was increased to induced RIR model. Pax6 expression was assessed by immunohistochemistry. Main Outcome Measures The expression of pax6. Results The positive rate of pax6 in RGC was （1.28±1.41） % in control group, （0.99±1.23）% in lw group, （14.45±2.72）% in 2w group, （50.88±4.73）% in 4w group, （71.00±4.72）% in 6w group and （78.80±4.62） % in 8w group. The difference of expression of pax6 in RGC among these groups was significant （F=1.350, P〈0.0001）. Compared with control group, the expression of pax6 in RGC was significant higher in 2w, 4w, 6w and 8w group （all P〈0.0001）, but not significant different in lw group （P=-0.835）. Conclusion Pax6 expression was present in every layer of retina after RIR injury. RIR in rat induced activation of endogenous retinal stem cells.