氢饱和生理盐水对视神经挫伤大鼠闪光VEP的影响

【摘要】 目的 探讨氢饱和生理盐水对视神经挫伤大鼠视觉功能的保护作用。设计 实验性研究。研究对象 健康成年雄性SD大鼠12只。方法 12只SD大鼠随机分为氢饱和生理盐水组和对照组，每组6只，均选取左眼作为实验眼，右眼作为正常对照。利用压力恒定的无创反向镊在大鼠视神经球后2 mm处夹持10 s建立视神经挫伤模型。氢饱和生理盐水组大鼠按5 ml/kg/d氢饱和生理盐水腹腔注射，对照组大鼠按照相同剂量给予生理盐水腹腔注射，连续2周。在夹伤前及夹伤后1、3、5、7、9、11、14天分别检测大鼠左眼F-VEP，观察P1波的峰时值及振幅的变化。主要指标 F-VEP的P1波峰时值和振幅。结果  损伤后第1天，P1波峰时值氢饱和生理盐水组和对照组分别为（88.61±2.81）ms和（88.33±1.51）ms，与损伤前的（72.45±1.47）ms和（72.44±1.03）ms相比，差异均有统计学意义（P均＜0.01）。损伤后第3天，氢饱和生理盐水组大鼠的P1波峰时值缩短为（80.28±1.25）ms，到损伤后14天一直在该水平范围波动，而对照组大鼠的P1波峰时值为（88.61±1.20）ms，各时期的氢饱和生理盐水组大鼠与对照组大鼠的P1波峰时值相比明显缩短，差异均有统计学意义（P均＜0.001）。  结论 氢饱和生理盐水能够改善大鼠视神经挫伤后F-VEP的峰时值延长，对其视觉传导功能起到一定的保护作用。（眼科， 2012, 21： 409-413）

Effect of hydrogen-rich saline on flash-VEP after the optic nerve crush in rats

Objective To investigate the protective effect of hydrogen-rich saline （HRS） on visual function of the rats undergone the optic nerve crush. Design Experimental study. Participants Healthy adult male SD rats. Methods 12 SD rats were randomly divid- ed into the HRS group （n=6） and the control group （n=6）. All the left eyes of the two groups were selected as the experimental eyes, and the right eyes served as normal controls. The optic nerve crush model was established by using a non-invasive forceps with a constant clipping pressure of 148 g to crush the exposed left optic nerves （ON） of the rats for l0 seconds at 2 mm behind the optic disc. After crush model was completed, the rats of the HRS group were given a Hydrogen-Rich Saline treatment by intraperitoneal injection with a dose of 5 ml/kg/d for consecutive two weeks, and the rats of the control group were given saline by intraperitoneal injection with the same dose. The F-VEP of the rats＇ left eye was detected at eight time points： pre-injury, 1 day （d）, 3 d, 5 d, 7 d, 9 d, 11 d, 14 d after injury. The changes of P1 wave peak time and amplitude were recorded. Main Outcome Measures The peak time and amplitude of the P1 wave. Results At the first day after injury, the peak time of the P1 wave in both groups （88.61±2.81 ms and 88.33±1.51 ms in the HRS group and the control group respectively）were significantly extended compared to the pre-injury （72.45±1.47 ms and 72.44±1.03 ms in the HRS group and the control group respectively）. The difference was statistically significant （all P〈0.01）. On the third day after injury, the peak time of the P1 wave in the HRS group was shortened to （80.28±1.25）ms, and fluctuated at this lever until 14 days post-injury. The peak time of the P1 wave in the control group remained prolonged （88.61±1.20 ms）. The difference between the two groups was statistically significant at each time point starting from the third day after injury （all P〈0.001）. Conclusions Hydrogen-rich saline can improve the extended peak time of the rats after optic nerve crush, and may play a protective role in the visual conductive system.