桔梗水提液的镇咳、祛痰作用研究

目的:研究桔梗水提液的镇咳、祛痰作用。方法:通过氨水引咳,观察小鼠咳嗽潜伏期和咳嗽次数,实验分为5组,即模型(等容生理盐水)、右美沙芬(0.03g·kg-1)和桔梗水提液高、中、低剂量(16、8、4g·kg-1)组,ig给药,每12h1次,连续6次。通过测定小鼠气管酚红排泌量来评定小鼠气管黏液分泌量,实验分为5组,即空白对照(等容生理盐水)、盐酸氨溴索(0.03g·kg-1)和桔梗水提液高、中、低剂量(16、8、4g·kg-1)组,ig给药,每12h1次,连续6次。结果:与模型组比较,桔梗水提液高、中剂量组咳嗽潜伏期显著延长(P<0.01或P<0.05),咳嗽次数显著减少(P<0.01或P<0.05)。与空白对照组比较,桔梗水提液高、中剂量组小鼠气管酚红排泌量显著增加(P<0.01或P<0.05)。结论:桔梗水提液在镇咳、祛痰方面效果较好,本研究可为临床用药提供理论依据。

Study on Antitussive and Expectorant Effect of Water Extract of Platycodon grandiflorum

OBJECTIVE： To study the antitussive and expectorant effect of water extract of Platycodon grandiflorum. METHODS： Through the ammonia led cough, cough incubation period and cough times in mice were observed; the experiment was divided into 5 groups, i.e. model group （constant volume normal saline）, dextromethorphan group （0.03 g·kg^-1）, water extract of P. grandiflorum high-dose, medium-dose and low-dose groups （16, 8, 4 g·kg^-1）. Those groups were given medicine intragastricaUy every 12 hours for 6 times. Through the IP phenol red, the secretion of trachea phenol red in mice was determined to evaluate the secretion of trachea mucus; the experiment was divided into 5 groups, i.e. blank control group （constant volume normal saline）, ambroxol hydrochloride （0.03 g·kg^-1） group, water extract of P. grandiflorum high-dose, medium-dose and low-dose groups （16, 8, 4 g·kg^-1）. Those groups were given medicine intragastrically every 12 hours for 6 times. RESULTS： Compared with model group, cough incubation period prolonged significantly in water extract of P. grandiflorum high-dose and medium-dose groups （P〈 0.01 or P〈0.05）, cough number significantly reduced （P〈0.01 or P〈0.05）. Compared with blank control group, the secretion of phenol red in water extract of P. grandiflorum high-dose and medium-dose groups increased significantly （P 〈 0.01 or P〈0.05）. CONCLUSION： Water extract of P. grandiflorum is effective for relieving cough and removing sputum. The study can provide theoretical basis for clinical medication.