钨酸钠对泊洛沙姆407诱导高血脂昆明小鼠模型血脂的影响

【目的】研究钨酸钠对泊洛沙姆407诱导的高血脂昆明小鼠模型血脂的影响。【方法】昆明小鼠给予钨酸钠（100或200mg/kg）灌胃,连续3 d,末次给药1 h后,腹腔注射泊洛沙姆407 0.3 g/kg,于注射后的4、24及48 h,取血测定甘油三酯和胆固醇含量,注射泊洛沙姆407后24 h血样同时测定高密度脂蛋白-胆固醇的含量。【结果】与正常组的甘油三酯（6.30±0.87）mmol/L相比,泊洛沙姆407腹腔注射4 h后,诱导昆明小鼠出现明显的高甘油三酯血症（233.99±37.94）mmol/L,预先给予钨酸钠处理,能明显降低血清甘油三酯水平[低剂量钨酸钠组：（207.13±29.85）mmol/L;高剂量钨酸钠组：（131.50±46.07）mmol/L,P〈0.05],此作用可持续到造模后的48 h。造模后的24 h,钨酸钠的降血清胆固醇的作用最明显[对照组：（6.07±0.70）mmol/L;低剂量钨酸钠组：（3.04±0.40）mmol/L;高剂量钨酸钠组：（1.81±0.40）mmol/L,P〈0.05]。造模后的24 h,预处理钨酸钠能明显升高血清高密度脂蛋白-胆固醇水平[对照组：（1.97±0.40）mmol/L;低剂量钨酸钠组：（2.70±0.42）mmol/L;高剂量钨酸钠组：（3.73±0.81）mmol/L,P〈0.05]。【结论】钨酸钠对于泊洛沙姆407诱导的高血脂昆明小鼠模型具有降脂作用。

Effects of sodium tungstate on poloxamer 407-induced hyperlipidemic Kunming mouse model

[Objective] To investigate the effects of sodium tungstate on serum lipids level in poloxamer 407-induced hyperlipidemic mouse. [ Methods ] Before a bolus of poloxamer 407 （0.3 g/kg） was given intraperitoneally, Kunming mice were pretreated intragastrically with sodium tungstate （100 or 200mg/kg） for 3 days. Blood triacylglycerol （TG） and cholesterol （CHO） were measured at 0, 4, 24 and 48 h after the poloxamer 407 injection, and the blood high density lipoprotein cholesterol （HDL-CHO） level of 24 h were measured. [ Results] Compared to normal group （6.30 ± 0.87） mmol/L, poloxamer 407 induced marked hypertriglyceridemia in Kunming mice （233.99 ± 37.94） mmol/L after intraperitoneal injection for 4 h. Pretreatment with sodium tungstate, could significantly reduce serum triglyceride levels （low dose of sodium tungstate group： （207.13 ± 29.85） mmol/L, high dose sodium tungstate group： （131.50 ± 46.07） mmol/L, the hypolipidemic effect of sodium tungstate is still obvious after 48 h （P 〈 0.05）. The most obvious lowering cholesterol of sodium tungstate can be observed after building the model of 24 h [control group： （6.07 ± 0.70） mmol/L; low dose of sodium tungstate group： （3.04 ± 0.40） mmol/L; high doses of sodium tungstate group： （1.81 ± 0.40） mmol/L, P 〈0.05]. After building the model of 24 h, pretreatment with sodium tungstate group can obviously increase the serum HDL-CHO [control group：（1.97 ± 0.40） mmol/L; low dose of sodium tungstate group： （2.70 ± 0.42） mmol/L, high doses of sodium tungstate group： （3.73 ± 0.81） mmol/L, P 〈 0.05]. [Conclusion] Sodium tungstate may have a hypolipidemic effects on poloxamer 407-induced hyperlipidemic Kunming mice and represent a potentially useful approach for the treatment of lipid metabolic disturbances.