核黄素缺乏大鼠血浆与红细胞核黄素及衍生物浓度的变化

目的:探讨核黄素缺乏大鼠血浆与红细胞核黄素及其衍生物浓度动态变化,为用血浆与红细胞核黄素及其衍生物水平评价核黄素营养状况提供依据。方法:以无核黄素饲料喂养4w造成大鼠核黄素缺乏,第5w改喂含核黄素的正常饲料。用高效液相色谱法测定血浆与红细胞核黄素及衍生物浓度的动态变化,同时测定核黄素尿排量、全血谷胱甘肽还原酶活性系数(BGRAC)、红细胞还原性谷胱甘肽浓度及血浆丙二醛浓度变化。结果:核黄素缺乏后血浆核黄素水平迅速下降,随之其衍生物水平也显著下降;红细胞核黄素及其衍生物水平变化晚于血浆的变化;BGRAC也有显著变化,但幅度较小;核黄素缺乏导致其尿排量也呈大幅度迅速下降趋势。改喂含核黄素正常饲料1w后,上述变化呈不同程度恢复。结论:血浆与红细胞核黄素浓度可作为评价核黄素营养状况的灵敏指标,需进行人体试验验证。

THE DYNAMIC CHANGES OF PLASMA AND ERYTHROCYTE RIBOFLAVIN AND ITS DERIVATIVES CONCENTRATIONS IN RIBOFLAVIN DEFICIENT RATS

Objective: To investigate the dynamic changes of plasma and erythrocyte riboflavin and its derivatives concentrations after riboflavin deficiency and so as to provide theoretic basis for their application in riboflavin status assessment. Methods: The riboflavin deficiency was induced in rats by feeding a riboflavin deficient diet. The plasma and erythrocyte riboflavin and its derivatives (FMN and FAD) were analysed with HPLC method. The blood glutathione reductase activity coefficient (BGRAC), erythrocyte GSH concentration and urinary riboflavin excretion were also measured. Results: The plasma riboflavin concentration as well as the urinary riboflavin excretion were decreased rapidly early after riboflavin deficiency and followed by plasma riboflavin derivatives. The erythrocyte riboflavin and its derivatives and GSH concentrations were decreased significantly later. On the other hand, the BGRAC was increased significantly in the early period of riboflavin deficiency and the change, however, was limited in a narrow range. Conclusion: The plasma and erythrocyte riboflavin concentration can be applied as a marker in riboflavin status assessment but human studies are necessary for further research.