| 蝮蛇毒磷酸二酯酶抗原的制备及性质 |
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| 引用本文： | 黄美华,顾肃敏,杨帆,吴益民,马洁羽.蝮蛇毒磷酸二酯酶抗原的制备及性质[J].浙江大学学报(医学版),1990(6). |
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| 作者姓名： | 黄美华 顾肃敏 杨帆 吴益民 马洁羽 |
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| 作者单位： | 浙江医科大学生物学教研室，浙江医科大学生物学教研室，浙江医科大学生物学教研室，浙江医科大学生物学教研室，浙江医科大学肿瘤基因研究组 |
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| 摘 要： | 为研究蛇类毒腺细胞分化过程中磷酸二酯酶的作用,需制备特异性和敏感性较高的该酶单克隆抗体用于免疫组化研究.本文所介绍为浙江蝮蛇蛇毒中磷酸二酯酶抗原的提取纯化过程,为制备单克隆抗体提供条件.作者采用离子交换技术和分子筛过滤法相结合的方法提取抗原,具有步骤少、分离效率高的特点.所得抗原经聚丙烯酰胺凝胶圆盘电泳呈单一条带,测定分子量为20900.
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| 关 键 词： | 蛇毒类 磷酸二酯酶类 抗原/生物合成 |
ISOLATION, PURIFICATION AND CHARA-CTERISTICS OF VENOM PHOSPHO-DIESTERASE ANTIGEN |
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| Huang Meihua,Gu Sumin.ISOLATION, PURIFICATION AND CHARA-CTERISTICS OF VENOM PHOSPHO-DIESTERASE ANTIGEN[J].Journal of Zhejiang University(Medical Sciences),1990(6). |
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| Authors: | Huang Meihua Gu Sumin |
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| Institution: | Huang Meihua,Gu Sumin,Department of Biology Ma Jiyu Tumor and Oncogene Research Group |
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| Abstract: | In order to study the effects of phosphodiesterase during the differentiation of venom gland cells by means of immunohistochemical techniques, the antigen with the phosphodiesterase activity from the venom of the snake Agkistrodon blomhoffii brevicaudus was prepared. The antigen was purified from the venom through ion-exchange chromatograph by using DEAE-Sephadex A-50, gel filtration on Sephadex G-100. The antigen shows the activity of phosphodiesterase (as measured with bis-4-nitrophenylphosphate as substrate). The homogeneity of the antigen was exhibited by polyacrylamide gel electrophoretic criterion. Its molecular weight is 20900 Dalton, as determined in SDS polyacrylamide gel electrophoresis. It might be helpful in developing monoclonal antibody. |
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| Keywords: | Snake venoms Phosphodiesterases Antigen/biosyn |
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