| 单核细胞骨架系统的电镜观察及胶体金免疫标记 |
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| 引用本文： | 陈晓梅,郭玉华,殷志伟,茅子均. 单核细胞骨架系统的电镜观察及胶体金免疫标记[J]. 苏州大学学报(自然科学版), 1992, 0(1) |
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| 作者姓名： | 陈晓梅 郭玉华 殷志伟 茅子均 |
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| 作者单位： | 苏州医学院病理生理学教研室，苏州医学院病理生理学教研室，苏州医学院病理生理学教研室，苏州医学院病理生理学教研室 硕士研究生，导师 |
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| 摘 要： | 应用整装细胞电镜技术结合选择性抽提方法,观察正常人外周血单核细胞的细胞骨架系统,并应用抗α管蛋白单克隆抗体和抗肌动蛋白抗体—胶体金免疫标记技术,分别对其胞浆中微管、微丝进行了定性研究。
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| 关 键 词： | 单核细胞 细胞骨架 整装细胞电镜 选择性抽提 胶体金免疫标记 |
Observation on the Sytoskeleton of Human Paripheral Blood Monocytes by Means of Using Mount Cell EM and Immunoglod Probes |
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| Chen Xiaomei,et al. Observation on the Sytoskeleton of Human Paripheral Blood Monocytes by Means of Using Mount Cell EM and Immunoglod Probes[J]. Suzhou University Journal of Medical Science, 1992, 0(1) |
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| Authors: | Chen Xiaomei et al |
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| Abstract: | Cytoskeletal framewok prepared by the selective extraction method has been observed withwhole mount cell electron microscopy.Cytoskeleton prepared by Triton X-100 treatment from hu-man periphral blood monocytes appeared in the examination by means of microscopy as a highly or-ganized and interconnected three-dimensional matrix of different fibrous elements.Since such cy-toskeleton are open membrane-free system,individual fibrous organization can be identified byspecfic antibodies.An indirect immunogold procedure with assay of monoclonal anti-tubulin oranti-actin antibodies was applied to visualize tubulin or actin containing structure.By further ex-traction with 0.25M(NH_4)_2SO_4 to remove the microtubules and microfilaments,only the salt-re-sistant intermediate filaments lattice were left. |
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| Keywords: | monocytes cytoskeleton system mount cells electron microscopy selective extraction method immunogold probes |
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