原发性高血压患者血小板聚集性与L-精氨酸转运功能的改变

为探讨高血压病患者血小板功能的改变及其可能的机制．本文观察了23名原发性高血压患者和14名健康成年人血小板体积、数量、聚集性和一氧化氮产生量及L-精氨酸转运特征。结果发现，原发性高血压患者血小板数目、体积无明显改变．而最大聚集率较对照组高1．3倍（P＜0．05），在二磷酸腺苷刺激下一氧化氮产生量较对照组低71％（P＜0．01），且血小板最大聚集率与一氧化氮产生量之间成明显负相关（r＝0．63）。原发性高血压患者血小板L-精氨酸转运能力明显降低，其最大转运速率仅为对照组的79％（P＜0．01），但两组间米氏常数无明显差异。结果提示，原发性高血压患者血小板聚集功能增强可能与一氧化氮生成减少及L-精氨酸转运功能降低有关。

Changes of Aggregation and L-Arginine Transport of Platelet in Patients with Essential Hypertension

Aim To study the changes of volume.function and characteristics of L-arginine(L-Arg)transport of platelet(Pt)in patients with essential hypertension (EH).Methods Nitric oxide(NO)production by platelets was assayed by measuring the accumulation of nitrite in the incubation medium using Greiss reaction accord-ing Green' method.Transport of 3H-L-Arg was determined essentially as described by William.In-flux of 3H-L-Arg was determined by measuring cell-associated radioactivity after an approprite period of in-cubation with 3H-L-Arg.Results in EH patients,the quantity and volume of Pt has no obvious difference with the normal.The maximum platelet aggregation ratio(PAR)was much higher than that in normal(P<0.05)and PAR was correlated with NO production.The production of NO and the ability of L-Arg transport in EH was re-spectively lower than that in normol(P<0.01).Maxi-mum transport velocity(Vmax)in EH was only 79%of normal(P<0.01).While the Michael constant (Km)showed no significant difference between the two groups.Conclusion Above results indicate that there exists a founctional and L-Arg transport disturbance in EH.It appears that the improvement of NO production and L-Arg transport in treatment of hypertension may be potentially important.