生育与不育男性精浆总抗氧化能力分析

目的:分析生育与不育男性精浆中总抗氧化能力(TAC)及其在男性生育中意义。方法:225例男性不育患者分为6组,分别为:梗阻性无精子症组(n=10),非梗阻性无精子症组(n=42),少精子症组(n=20),弱精子症组(n=78),少弱精子症组(n=57),以及正常精子症组(n=18)。28例正常生育男性作为对照(生育组)。分别采用计算机辅助精液分析(CASA)系统进行精液参数分析,采用比色法检测精浆TAC水平。结果:生育组男性精浆TAC为(19.82±6.33)U,梗阻性无精子症组(1.71±1.33)U,非梗阻性无精子症组(12.73±9.44)U,少精子症组(10.85±6.64)U,弱精子症组(13.88±8.24)U,少弱精子症组(11.20±7.02)U,正常精子症组(18.07±8.73)U;与生育组精浆TAC(19.82±6.33)U]相比,在各不育症组中,除正常精子症组精浆TAC与生育组差异无显著性外,其余各组均显著低于生育组(P<0.01)。精浆TAC与精子密度(r=0.182,P<0.05)和a级精子(r=0.150,P<0.05)呈显著正相关。结论:精浆中TAC水平与男性不育密切相关,精浆中过低的TAC水平可能是引起男性不育的病因之一。

Total Antioxidant Capacity of Seminal Plasma in Fertile and Infertile Men

Objective: To evaluate total antioxidant capacity(TAC) of seminal plasma in fertile and infertile men and understand the relation between seminal plasma TAC and male fertility.Methods: Two hundred and twenty-five infertile men were divided into 10 cases of obstructive azoospermic men,42 cases of non-obstructive azoospermic men,20 cases of oligozoospermic men,78 cases of asthenozoospermic men,57 cases of oligoasthenozoospermic men,and 18 cases of normozoospermic men,then 28 fertile men were taken as the control.The seminal parameter analysis was performed by computer-assisted semen analysis(CASA) system.Seminal plasma TAC was measured using spectroscopic analysis.Results: Seminal plasma TAC were((1.71)±(1.33))U in obstructive azoospermic men,((12.73)±(9.44)) U in non-obstructive azoospermic men,((10.85)±(6.64)) U in oligozoospermic men,((13.88)±(8.24)) U in asthenozoospermic men,((11.20)±(7.02)) U in oligoasthenozoospermic men,((18.07)±(8.73)) U in normozoospermic men, and((19.82)±(6.33)) U in fertile men.There was nosignificant difference in TAC between normozoospermic men and fertile men(P>(0.05)).Compared with fertile men,seminal plasma TAC in other infertile groups was significantly lower(P <(0.01)).There were significantly made positive correlation beteen seminal plasma TAC and sperm density(r=(0.182),P<(0.05)),as well as sperm with grade a(r=(0.150),P <(0.05)).Conclusion: Seminal plasma TAC is closely related to male fertility,and the decreased level of TAC in seminal plasma may be one of the causes of male infertility.