Therapeutic time window for the neuroprotective effects of NGF when administered after focal cerebral ischemia |
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Authors: | Ji-Ping Yang Huai-Jun Liu Hua Yang Ping-Yong Feng |
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Institution: | (1) Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, Shijiazhuang, 050000, Hebei Province, China; |
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Abstract: | In the present study, we evaluated the neuroprotection time window for nerve growth factor (NGF) after ischemia/reperfusion
brain injury in rabbits as related to this anti-apoptosis mechanism. Male New Zealand rabbits were subjected to 2 h of middle
cerebral artery occlusion (MCAO), followed by 70 h of reperfusion. NGF was administered after injury to evaluate the time
window. Neurological deficits, infarct volume, neural cell apoptosis and expressions of caspase-3 and Bcl-2 were measured.
Compared to saline-treated control, NGF treatment at 2, 3 and 5 h after MCAO significantly reduced infarct volume, neural
cell apoptosis and expression of caspase-3 (P < 0.01), up-regulated the expression of Bcl-2 and improved functional recovery (P < 0.01). However, treatment at latter time points did not produce significant neuroprotection. Neuroprotection treatment
with NGF provides an extended time window of up to 5 h after ischemia/reperfusion brain injury, in part by attenuating the
apoptosis. |
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Keywords: | |
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