改良白膜法制备汇集浓缩血小板的临床应用

目的本研究旨在改进手工制备浓缩血小板方法，提高血小板制备质量。方法在白膜法的基础上，延长血小板解聚时间，汇集多人份白膜层，第2次离心时采用二元无聚梯度离心原理，收集血小板。结果7份按本法制备的汇集浓缩血小板（10U／袋），其血小板计数、血小板回收率及白细胞、红细胞混入量、容量分别为（2．90±0．32）×10^11、（66±4）％、（4．2±1．9）×10^8／袋、（7．2±2．3）×10^9／袋、250～300ml。结论本法制备的手工血小板回收率较高，质量指标全部超过全血和成分血国家质量标准，适宜血站推广应用。

Collect Abcurrent Platelet Concentrates by Improved Bully Coat

Objective The aim is to improve the method of hand-collected platelet concentrate,and to elevate its qulity. Methods On the basis of bully coat collected platelet concentrates（BC PC）, we attempted to prolong the depolymerization time and collect multi-units bully coat. The contrifugalization condition for platelet collection was also greatly improved in dimension step run. Results The platelet concentration, recovery, residual leukocyte and residual erythrocyte,volume were （2.90±0.32） × 10^11, （66±4） %, （4.2±1. 9）× 10^8/package, （7.2±2.3） × 10^9/package, （274±27） ml in 7 packs. Conclusion The results showed that the hand-collected platelet concentrates exceed the standard of the national quality, its recovery is high, and no other device is needed. The method can be generalizaed for its easy operation and quality.