| 肉毒素和肿瘤坏死因子对大白鼠肝功能的影响 |
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| 引用本文： | 曹显科,张蒙. 肉毒素和肿瘤坏死因子对大白鼠肝功能的影响[J]. 中国组织工程研究与临床康复, 2002, 6(10) |
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| 作者姓名： | 曹显科 张蒙 |
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| 作者单位： | 1.107Central Hospital of PLA，Yanta i 264002，China;2.Central Hospital of Xi ' an，Xi ' an 710000，China |
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| 摘 要： | １MaterialsandMethods１．１ExperimentalanimalsSDmaleratswiththeageof１monthproducedinShanghai，weightedfrom９０gto１００g．１．２MainreagentsandinstrumentsDMEMmedium（providedbyGIBCOcompanyofUSA），０．０３％collagenaseⅠsolutions５０ml（SigmaCorporation，withDMEMassolvent），phosphoricbuffersolution（PBS）２５０ml，RDB－７８peristalsispump（InstrumentCorporationofZhangjiaganginJiangsu），cen－trifugalapparatusofBiofuge１５R，productionofGermanycompa－ny．１．３Separationandcultu…
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The effect of endotoxin and tumor necrosis factor on hepatic function of rats |
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| CAO Xian-ke ,ZHANG Meng. The effect of endotoxin and tumor necrosis factor on hepatic function of rats[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2002, 6(10) |
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| Authors: | CAO Xian-ke ZHANG Meng |
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| Affiliation: | 1. 107 Central Hospital of PLA,Yantai 264002,China
2. Central Hospital of Xi'an, Xi'an 710000,China |
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| Abstract: | Objective To investigate the effect of endotoxin and tumor necrosis factor (TNF) on hepatic function of rats. Method Prepare hepatic cell suspension by filling male SD rats' livers with collagenaseⅠ ,culture in DMEM medium, and add endotoxin and TNF in it. After 24 hours detect GPT, GOT in supernatant fluid. Result The activity of GPT and GOT in supernatant fluid increased after adding endotoxin and TNF . Conclusion The system of hepatic cell membrane could be damaged severely after acted by endotoxin and TNF. |
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| Keywords: | endotoxin tumor necrosis factor h epatic function |
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