| pSVPoMCAT微基因修饰雪旺氏细胞移植治疗实验性脊髓损伤的酶及免疫组化评价 |
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| 引用本文： | 陈礼刚,朴永旭,曾凡俊,高立达,毛伯镛,杨立斌,黄茂清,胡威夷.pSVPoMCAT微基因修饰雪旺氏细胞移植治疗实验性脊髓损伤的酶及免疫组化评价[J].中国组织工程研究与临床康复,2002,6(12). |
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| 作者姓名： | 陈礼刚 朴永旭 曾凡俊 高立达 毛伯镛 杨立斌 黄茂清 胡威夷 |
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| 作者单位： | 1.Chinese PLAChengdu General Hospital of Chendu Military Command Area，Chengdu 610083 |
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| 摘 要： | Genetherapyofspinalcordinjury（SCI）remainsintheexper－imentalstage．Now，neurotrophicfactortomodifySchwanncell（SC）orfibroblastsareimplantedintoSCIregiontoobservetheregenera－tionsofaxons犤１犦．WefirstbuildPo－５＇－flankinginducedmicrogeneforbasicproteinofmyelinsheathwhichistransientlynamedaspSVPoMcat．ThepSVPoMcatgenewasintroducedintoSCthroughcationliposome．ItwasprovedthatpSVPoMcatcouldenhancefunc－tionofSCandelongateitssurvivalperiodinvitro犤２犦．pSVPoMcatwasimplantedintoS…
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Microgene pSVPoMcat implanted to modify Schwann cell and recovery of function after spinal cord injury |
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| CHEN Li-gang ,PIAO Yong-xu ,ZENG Fan-jun ,GAO Li-da ,MAO Bo-yong ,YANG Li-bin ,HUANG Mao-qing ,HU Wei-yi.Microgene pSVPoMcat implanted to modify Schwann cell and recovery of function after spinal cord injury[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2002,6(12). |
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| Authors: | CHEN Li-gang PIAO Yong-xu ZENG Fan-jun GAO Li-da MAO Bo-yong YANG Li-bin HUANG Mao-qing HU Wei-yi |
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| Institution: | 1. Chinese PLA Chengdu General Hospital of Chendu Military Command Area,Chengdu 610083,China
2. First Affiliated Hospital of West China University,Chengdu 610041,China |
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| Abstract: | Objective To investigate the effect of microgene pSVPoMcat to modify genetically Schwann cell(SC) on recovery of function after spinal cord injury(SCI).Methods Experimental animals were divided into three groups:the group of microgene pSVPoMcat implanted to genetically(group A),SC implanted group(group B),and the control group(group C),each group having 10 animals.The cortical somatasensory evoked potentials(CSEP) and combined behavioral score(CBS) were continually monitored from operation second week to twelfth week.We used image analysis system to compare the latent period and peak amplitudes of CSEP and count the axon number among the different group.At the same time,electron microscope obsevation was conducted.Results The peak latency and amplitudes of group A,B showed a recovery tendency and it was constant with CBS.The number of regenerated axons of the group A and group B were (388± 163) and (109± 16),respectively(P < 0.01),in line with the observation of electron microscope.Conclusion We conclude that microgene pSVPoMcat to modify genetically SC plays a promoting role in recovery of central function after SCI. |
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| Keywords: | genetically modify Schwann cell i mplantation spinal cord injury |
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