去端肽猪皮Ⅰ型胶原在兔角膜组织中的生物相容性

目的 评价去端肽猪皮Ⅰ型胶原在兔角膜组织中的生物学反应,以探讨其作为角膜支架材料的可行性.方法 大耳白兔40只,分为5组,每组8只,一眼为实验眼,另一眼作为正常对照眼.将去端肽猪皮Ⅰ型胶原植入兔角膜前板层,术后1个月内每周2次、1个月后每月1次行肉眼、裂隙灯观察,并于每次观察后记录角膜是否透明、有无新生血管的评分情况;术后3 d、14 d、1个月、3个月及6个月,取兔角膜行组织病理学观察以及上皮细胞标志蛋白K3的免疫组化检测.评分结果采用Friedman秩和检验行组间比较,两两之间比较行Wilcoxon符号秩和检验.结果 术后角膜透明度逐渐恢复,新生血管化程度逐渐升高,至术后1个月最高,后逐渐减轻并退化;术后6个月,角膜透明度和新生血管化程度与正常眼比较,差异无统计学意义（P＞0.05）.整个观察期间未见角膜坏死、溃疡及植片脱出.结论 去端肽猪皮Ⅰ型胶原具有较好的生物相容性,进一步改进后有望成为一种新型的角膜移植支架材料.

Biocompatibility of porcine type Ⅰ atelocollagen transplanted in rabbit cornea

Objective To evaluate the biological response of biodegradable scaffold material porcine type Ⅰ atelocollagen in rabbit cornea in order to study the feasibility of corneal reconstruction using this material. Methods Forty rabbits were divided into 5 groups based on observation time with 8 rabbits in each group. One eye was used as the experimental eye, the other eye was used as the normal control eye. Scaffold material was transplanted into the anterior lamellar of rabbit corneas.Observations were performed with the naked eye and slit lamp biomicroscopy twice a week after the operation within 1 month, and after then, observations were performed once a month. Transparency and neovascularization scores of the cornea were recorded. HE histological examinations were done on the day 3, day 14 and the 1st, 3rd and 6th month, and the immunohistochemistry of the epithelial cell marker protein K3 was assessed. Statistical methods were used to test for related samples and to test for two comparison samples. Results The transparency of the cornea gradually increased (P＜0.05). The extent of corneal neovascularizazion gradually increased, reached a peak in the 1st month,and then gradually decreased (P＜0.05). The difference was not statistically significant when the transparency and neovascularization of the cornea 6 months postoperatively were compared to normal eyes (P＞0.05). Conclusion The scaffold material has better biocompatibility, and will become a new scaffold material for corneal transplants after further improvement.