球形幽门螺杆菌生物学性状及蛋白产物研究球形Hp系列研究之二

目的：研究球形幽门螺杆菌生物学性状和蛋白产物发生的改变。方法：通过延长培养时间和亚抑菌浓度抗生素－－灭滴灵的作用，分别获得两类球形幽门螺杆菌（Ｈｐ）。结果：两种不利于Ｈｐ生长的环境在引起Ｈｐ发生球变的同时，每毫升菌落形成单位（ＣＦＵ／ｍｌ）减少，尿素酶活性降低。结论：球形Ｈｐ尿素酶活性低下，且在常规培养基上不能生长。抗生素的作用比延期培养更快使Ｈｐ发生球形变异。聚丙烯酰胺凝胶电泳蛋白质条带分析发现抗生素诱变的球形Ｈｐ与正常形态Ｈｐ基本相似，与延期培养的Ｈｐ明显不同。

Biological Characteristics and Protein Products of Helicobacter Pylori

Objective:Biological characteristics and proteinic bands of two kinds of coccoid H.pylori,which were obtained by prolonged incubation or exposing in sub-MIC of metronidazole,were included in this study.The morphological conversion of H,pylori in unfavorable conditions was paralleled by decrease of colony-forming units per milliliter(CFU/ml)and tirease activity.Results:That coccoid H.pylori with low urease activity could not grow in routine growth media.It took less time to convert coccoid H,pylori by antibiotics than by prolonged culture.Conclusion:That proteinic bands were similar between bacillary and antibiotic-induced coccoid H.pylori,but quite different between long-culttired and antibiotic-induced coccoid H.pylori.