肺癌组织中糖胺多糖含量和硫酸化特点研究

目的：探讨组织中糖胺多糖与肿瘤发生的关系。方法：应用自行提纯的硫酸软骨素裂解酶和高效液相色谱（ＨＰＬＣ）二糖分析方法，从细胞外基质代谢的疾病生化学角度，对肺癌患者的病变组织及体液糖胺多糖（ＧＡＧ）进行了组分和性质的研究。结果：表明肺癌患者组织ＧＡＧ总量明显高于癌旁的非癌组织；肺癌组织ＧＡＧ表现为高硫酸化。肺癌患者尿ＧＡＧ排泄量降低，ＧＡＧ组分呈严重低硫酸化，ＧＡＧ的二糖中非硫酸化二糖（△Ｄｉ ０Ｓ）含量高于健康对照组１５倍。结论：细胞外基质ＧＡＧ并非仅起单纯的支持和保护作用，还与细胞分化、增殖及其代谢调节等多种生理功能有关。ＧＡＧ代谢异常与肿瘤的发生、发展过程密切相关

Study on composition and properties of glucosamineglycan in lung cancer

Objective:In order to understand the metabolism of carcinomas.Methods:GAG contents and composition of lung cancer from 5 patients were studied.Results:When the GAG quantity in neoplastic tissues was compared with that in the normal appearing tissues surrounding the neoplasms;tatal GAG contents ranged from 1.04 to 5 times the normal appearing tissues surrounding the carcinomas.Sulfated dissacharides increased in the GAG of neoplastic tissues.The GAG of neoplastic tissues were oversulfated.Total GAG of unrine from neoplastic patients reduced.△ Di 0S content increased about 15 times in urinary GAG of the neoplastic patients.The GAG was serious undersulfated.Conclusion:The results demonstrated that the abnormal metabolism of GAG was closely related to origination and development of neoplasm.