| 高效液相色谱法测定工人尿中三硝基甲苯及五种代谢物(一)——色谱分离及水解、提取条件的选择 |
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| 引用本文： | 姚明,缐引林.高效液相色谱法测定工人尿中三硝基甲苯及五种代谢物(一)——色谱分离及水解、提取条件的选择[J].卫生研究,1989(4). |
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| 作者姓名： | 姚明 缐引林 |
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| 作者单位： | 中国预防医学科学院劳动卫生与职业病研究所，中国预防医学科学院劳动卫生与职业病研究所 |
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| 摘 要： | 本文用高效液相色谱法在5μm反相C~(13)离子对柱、甲醇/水系统下,分离了TNT及五种代谢物。确定了尿样的水解采用两种方式:在40℃下加6N HCl(0.1ml/ml尿)水解10分钟,中和后提取4-HA;在100℃下加浓HCl(0.1ml/ml尿)水解60分钟,中和后提取其它TNT代谢物。提取剂用苯,提取比为1:1,溶液pH=7,振荡时间5分钟。在上述水解条件下可使各代谢物水解量达到最大。
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| 关 键 词： | 三硝基甲苯 代谢物 高效液相色谱 尿 |
Determination of TNT and its Five Metabolites in Urine of Workers by High Performance Liquid Chromatography(HPLC) (1) Selection of Conditions for Chromatographic Separation, Hydrolization and Extraction |
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| Yao Ming and Xian Yinlin.Determination of TNT and its Five Metabolites in Urine of Workers by High Performance Liquid Chromatography(HPLC) (1) Selection of Conditions for Chromatographic Separation, Hydrolization and Extraction[J].Journal of Hygiene Research,1989(4). |
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| Authors: | Yao Ming and Xian Yinlin |
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| Abstract: | In this paper, TNT and its five metabolites were separated under the system of 5μm reversed-phase C18 ion-pair column and methanol-water by HPLC. Two diffierent ways were used for hydrolization of metabolites in urine. Hydrolization condition of 4-HA in urine is that urine was acidified with 6N hydrochloric acid(0.1ml/ml urine)and heated at 40℃ for 10 min. Hydrolization condition of other metabolites is that urine was acidified with concentrated hydrochloric acid(0.1 ml/ml urine)and heated at 100℃ for 60 min. Benzene was selected as the extracted solvent, the ratio of two phase was 1 : 1, pH=7,extracted by shaking 5 min. Through the above-mentioned hydrolization condition, maximum degree of hydrolization could be obtained. |
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| Keywords: | TNT metabolite HPLC urine |
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