改良纸片扩散法检测172株酵母菌对氟康唑的敏感性

目的 研究氟康唑对172株酵母菌的体外抗菌活性。方法 酵母菌收集自2004年4月至2004年10月从住院患者采集的标本。采用NCCLS建议的改良纸片扩散法敏感试验，并以标准菌株做质量控制。结果 白念珠菌最常见，有110株，占64．0％；光滑念珠菌31株，占18．0％；热带念珠菌20株，占11．6％；克柔念珠菌3株，占1．8％；其他8株，占4．6％。白念珠菌、光滑念珠菌、热带念珠菌和克柔念珠菌对氟康唑的敏感率为92．3％～100％。结论 氟康唑对白念珠菌、光滑念珠菌、热带念珠菌和克柔念珠菌有较好的抗菌活性。

Susceptibility of 172 strains of yeast to fluconazole tested by modified disk diffusion method

Objective To mvestigate the in vitro activity of fluconazole 172 strains of yeast. Methods The yeasts were isolated from in-patlents of 4th Hospital of Jilin University from April 2004 to October 2004. Susceptibility testing was performed using a modified disk diffusion method which is recommended by NCCLS M2-A6. All the tests were controlled by reference strains. Results C. albicans were the most common isolates which accounted for 64. 0% of all the isolates. C. glabrata, C. tropicalis, C. krusei and other species accounted for 18.0%, 11.6%, 1.8% and 4.6% respectively. The susceptibility rate of C. albicans, C. glabrata, C. krusei and C. tropicalis to fluconazole ranged from 92.3% to 100.0%. Conclusions Fluconazole has good in vitro activity against C. albicans, C. glabrata, C. krusei and C. tropicalis.