无偿献血人群人类免疫缺陷病毒抗体和核酸检测分析

目的　了解深圳地区无偿献血人群人类免疫缺陷病毒(HⅣ)感染流行情况。方法　采用酶联免疫吸附试验(ELISA)对无偿献血者进行抗-H1V-1／2筛查，对初查阳性标本用聚合酶链反应(PCR)方法　进一步检测外周血单个核细胞(PMBCs)中HIVDNA和血浆中HIVRNA，并对2例无偿献血者PCR扩增阳性片段进行序列测定。结果　在46095例无偿献血者中，发现抗-HIV1阳性7例，阳性率为0.015％；在7例抗-HIV-1阳性献血者的PBMCs中均检出HIVDNA阳性，5例血浆中检测出HIVRNA阳性；2例献血者HIVDNA测序分析显示，感染的是HIV-1E亚型毒株。结论　深圳地区无偿献血者人群存在HIVE亚型毒株感染，对无偿献血者仍应加强HIV感染的筛查和监测。

Detection of anti- HIV- 1/2 and HIV nucleic acid in population of non- remunerated blood donors in Shenzhen

Objective To study the prevalent status of HIV-1 in population of non-remunerated blood donors in Shenzhen.Methods 46 095 non-remunerated blood donors were tested for anti-HIV-1/2 by ELISA. The donors of anti-HIV positive were further detected for HIV DNA from peripheral blood mononuclear cells (PMBCs) by nested-PCR and HIV RNA from plasma by RT-PCR. The partial genome of env of 2 blood donors were sequenced.Results The anti-HIV-1 was tested positive in 7 of 46 095 voluntary blood donors and the positive rate was 0.015%. In these 7 non-remunerated blood donors of anti-HIV-1 positive,7 were positive for HIV DNA in PMBCs and 5 positive for HIV RNA in plasma. The sequence analysis showed that 2 donors were infected by HIV-1 subtype E strains.Conclusion There exists HIV-1 subtype E infection in population of non-remunerated blood donors in Shenzhen.It is essential to detect and monitor strictly the population of non-remunerated blood donors.