生物人工肝中肝细胞来源及培养的新进展

肝细胞的活性是生物人工肝成功的关键 ,而良好的细胞来源及培养是肝细胞活性的保证。近年来 ,生物型人工肝中肝细胞来源及培养有了很大的进展。其中 ,肝细胞来源的研究主要表现在原代肝细胞、人肝癌细胞、传代及转化的肝细胞等方面。肝细胞培养的研究集中在培养方式、培养条件、培养时间及所需肝细胞数量等方面。这些研究解决了许多理论和实践上的问题 ,促进了生物人工肝的发展

The new progress of cell source and cell culture in bioartifical liver

The activity of hepatocytes is the key point of the Bioartifical liver(BAL) to succed.And satisfatory cell source and cell culture could guarantee the hepatocyte's activity.Recently,human have make many advacements in cell source and cell culture of BAL.Now,the hepatocytes in BAL could be get from several ways,such as primary hepatocytes,liver cancer cells,conversioned hepatocytes,etc.There also are many advancements in cell culture,such as culturing styles,culturing conditions and culturing times,etc.These researchs are contributed to solve many problems in BAL and promote it's developments.