不同血清学标志物与HBV-DNA含量及Pre-S1在诊断乙肝病毒复制中的价值

目的了解乙型肝炎血清学不同标志物模式(HBV-M)与乙肝病毒DNA含量及乙肝病毒前S1蛋白的关系,为预测乙型肝炎病毒的传染程度与乙肝的预防、诊断及治疗提供实验室依据。方法采用美国ABBOTT公司生产的AXSYM全自动免疫分析仪和微粒子酶免检测试剂盒对530例携带不同血清学标志物的乙肝患者进行乙肝五项指标检测,并用聚合酶链反应定量检测血清HBV-DNA含量,同时,用ELS IA法检测乙肝病毒前S1抗原。结果五种HBV免疫模式中HB sA g,HB eA g和HB cA b同时阳性,其HBV-DNA定量阳性和HBV-P reS1的检出率分别为94.1%和92.8%;HB sA g,HB eA b和HB cA b阳性组的检出率分别为53.5%和40.1%;其它不同血清学模式组也有不同的阳性率。结论P re-S1可敏感反映乙型肝炎病毒的复制情况,尤其可反映HB eA g阴性的乙肝患者仍存在病毒复制。HBV-DNA含量与P re-S1呈正相关,HBV-DNA、乙肝病毒五项标志和前S1蛋白联合检测,对更好地早期诊断HBV感染,了解病毒的复制情况,疗效观察和预后判断均具有重要意义。

Clinical Signification of Diagnosis Various Immunological Modes of HBV and HBV-DNA and HBV-PreS1 Protein for Identifying Viral Replication in Patients with HBV

objective To study the relationship between theserological markers of hepatitis B and the quantity of HBV-DNA and HBV-PreS1.In order to provide laboratory basis for predicting the infectivity,prevention and treatment of hepatitis B.Methods 5 serological markers of hepatitis B were detected with Abbott AXSYM Immunoassay Analyzer and micro-particles enzyme immunoassay kits,HBV-DNA was determined by real-time quantitative PCR and HBV-PreS1 was measured with ELISA,from 530 hepatitis B patientswere analyzed in the study.Results The HBV-DNA and HBV-PreS1 positive rates in HBsAg( ),HBeAg( )and HBcAb( )specimens were 94.1% and 92.8% respectively.In HBsAg( ),HBeAb( )and HBcAb( )specimens were 53.5%and 40.1% respectively.The HBV-DNA and HBV-PreS1 positive rates varied in other.Conclusion HBV PreS1 protein can reflect the situation of viral replication more sensitively,especially for those who are HBeAg negative,The HBV-DNA contents relates positively with HBV-PreS1 positive rates on the whole.It is very important to monitor.The HBV-DNA,5 serological markers and HBV-PreS1 synchronous detection for the diagnosis of HBV infection in earlier,the investigation of reproduction,therapeutic observation and prognosis.