两种猪自体骨髓间充质干细胞培养方法的比较

目的比较猪应用单纯直接贴壁法和密度梯度离心结合直接贴壁法两种方法分离、纯化、扩增骨髓间充质干细胞(MSCs)的培养效率,为临床应用提供理论依据和实验方法。方法取滇南小型猪的骨髓液用单纯直接贴壁法和密度梯度离心结合贴壁培养法两种方法分离猪骨髓间充质干细胞,体外培养扩增,绘制其生长曲线,通过显微镜观察培养细胞的生物学形状。结果直接贴壁法收集到的细胞扩增速度较慢,密度梯度离心法收集到的细胞扩增速度较快,细胞培养后成梭形。结论密度梯度离心结合贴壁培养法较单纯的直接贴壁法培养骨髓间充质干细胞的培养效率更高。体外培养的猪骨髓间充质干细胞生长稳定,增殖力较强。

A comparative study on culturing swine bone marrow-derived mesenchymal stem cells by two methods

Objective To separate, purificate and amplify swine bone marrow-derived mesenchymal stem cells（MSCs） through two methods that direct adherent and density gradient, compared the cultivation efficiency of the two methods, so that to provide a theoretical basis for clinical application and experimental methods.Methods Diannan swine bone marrow was taken with two culture methods, i.e a simple direct adherent, and density gradient centrifugation combined with adherent, to separate swine bone marrow mesenchymal stem cells and amplificate in vitro,then the shape of cell was observed through the microscope and cell growth＂ curve was drawn. Results The proliferation speed of collected cells was slow by direct adherent, and the speed was faster by density gradient centrifugation, and the cell forms after the culture were spindle cells. Conclusion The culture speed of MSCs by density gradient centrifugation combined adherent is faster than that by a simple direct adherent. The growth of MSCs cultured in vitro is stable, and the proliferation potential is strong.