| ESTABLISHMENT OF A HUMAN T-LYMPHOMA CELL LINE（H-TL90） AND ANALYSIS OF ITS BIOLOGICAL CHARACTERISTICS |
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| 引用本文： | 史历,刘旭,张月梅,李有芳,李殿俊,王吾如.ESTABLISHMENT OF A HUMAN T-LYMPHOMA CELL LINE（H-TL90） AND ANALYSIS OF ITS BIOLOGICAL CHARACTERISTICS[J].中国医学科学杂志,1995,10(3):178-180. |
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| 作者姓名： | 史历 刘旭 张月梅 李有芳 李殿俊 王吾如 |
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| 作者单位： | [1]DepartmentofPathology,HarbinMedicalUniversity,Harbin150086 [2]HarbinpharraaeeuticalFactoryHospiud,Harbin1S0086 [3]不详,Harbin1S0086 |
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| 摘 要： | We established a human T-lymphoma cell line from the cancerous ascites of a male patient with prostate cancer which was named H-TL90. This cell line was characterized by its histological features, and by chromosomal and immunological analysis. Immunophenotypic analysis revealed that the cells expressed surface antigen CD3^- CD4^- CD7^ CD8^-. Biological analysis revealed that the cell can promote lymphocyte proliferation. This suggested that the cell line has an autosecretion function. Cytogenetic analysis revealed that H-TL90 was a hyperdlploid with 47 chromosomes and had characteristic translocation between chromosome 3 and 11, and the deletion of the long arm of chromosome 6. These results demonstrated the H-TL90 cell line can be a useful modal for the study of human T-lymphoma.
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| 关 键 词： | T淋巴细胞 细胞生物学 癌症 腹水 免疫组织染色 H-TL90细胞 |
ESTABLISHMENT OF A HUMAN T-LYMPHOMA CELL LINE(H-TL90) AND ANALYSIS OF ITS BIOLOGICAL CHARACTERISTICS |
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| Shi Li,Liu Xu,Zhang Yuemei,Li Xiufang, Li Dianjun and Wang Wuru.ESTABLISHMENT OF A HUMAN T-LYMPHOMA CELL LINE(H-TL90) AND ANALYSIS OF ITS BIOLOGICAL CHARACTERISTICS[J].Chinese Medical Sciences Journal,1995,10(3):178-180. |
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| Authors: | Shi Li Liu Xu Zhang Yuemei Li Xiufang Li Dianjun and Wang Wuru |
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| Institution: | Shi Li;Liu Xu;Zhang Yuemei;Li Xiufang; Li Dianjun and Wang Wuru |
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| Abstract: | We established a human T-lymphoma cell line from the cancerous ascrtes of a male patient with prostate cancer which was named H-TL90. This cell line was characterized by its histological features, and by chromosomal and immunological analysis. Immunophenotypic analysis revealed that the cells expressed surface antigen CD3- CD4- CD7 CD8-. Biological analysis revealed that the cell can promote lymphoryte proliferation. This suggested that the cell line has an autosecretion function. Cytogenetic analysis revealed that H-TL90 was a hyperdiploid with 47 chromosomes and had characteristic transloration between chromosome 3 and 11, and the deletion of the long arm of chromosome 6. These resuhs demonstrated the H-TL90 cell line can be a useful model for the study of human T-lymphoma. |
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| Keywords: | T-lymphoma cell line |
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