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| 北京地区哮喘儿童特异性IgE和TRPVl／2mRNA表达水平分析 | |
| 引用本文： | 朱泽轶,冯佳,李红,蔡欣,邹民吉,王强,王嘉玺,徐东群,徐东刚. 北京地区哮喘儿童特异性IgE和TRPVl／2mRNA表达水平分析[J]. 军事医学科学院院刊, 2011, 35(8): 616-619 |
| 作者姓名： | 朱泽轶 冯佳 李红 蔡欣 邹民吉 王强 王嘉玺 徐东群 徐东刚 |
| 作者单位： | 1. 军事医学科学院基础医学研究所,北京,100850 2. 北京大学第一医院,北京,100034 3. 中国疾病预防控制中心环境与健康相关产品安全所,北京,100021 |
| 基金项目： | 国家973计划项目(211CB5383); 国家科技支撑计划课题(26BAII9B5-3) |
| 摘 要： | 目的探讨TRPVl和TRPV2基因表达水平与北京地区儿童哮喘的关系。方法实时定量PCR方法检测38例健康儿童，46例哮喘儿童外周血淋巴细胞中TRPVl和TRPV2mRNA表达水平差异。免疫印迹法定量检测总IgE水平，尘螨、春季花粉、秋季花粉、蟑螂和猫狗毛、真菌等特异性TgE水平，对检测结果进行多因素lo~stic回归分析。结果健康和哮喘组儿童TRPVl和TRPV2的mRNA表达水平差畀有统计学意义（P〈0．01），真菌特异性IgE表达水平差异有统计学意义（P〈0．01）。结论TRPVl和TRPV2基因表达水平升高可能是北京地区儿童哮喘发病酌危险圆素。 |
| 关 键 词： | 儿童哮喘 TRPVl TRPV2 RNA 信使 特畀性IgE |
Analysis of specific IgE and TRPV1/2 mRNA in asthmatic children in Beijing |
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| ZHU Ze-yi,FENG Jia,LI Hong,CAI Xin,ZOU Min-ji,WANG Qiang,WANG Jia-xi,XU Dong-qun,XU Dong-gang. Analysis of specific IgE and TRPV1/2 mRNA in asthmatic children in Beijing[J]. Bulletin of the Academy of Military Medical Sciences, 2011, 35(8): 616-619 | |
| Authors: | ZHU Ze-yi FENG Jia LI Hong CAI Xin ZOU Min-ji WANG Qiang WANG Jia-xi XU Dong-qun XU Dong-gang |
| Affiliation: | ZHU Ze-yi1,FENG Jia3,LI Hong2,CAI Xin1,ZOU Min-ji1,WANG Qiang2,WANG Jia-xi1,XU Dong-qun2,XU Dong-gang1 (1.Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850,China,2.Institute of Environmental Health and Related Product Safety,Center for Disease Prevention and Control of China,Beijing 100021,3.The First Hospital of Peking University,Beijing 100034,China) |
| Abstract: | Objective To study the relationship between the expression levels of TRPV1 and TRPV2 genes and childhood asthma in Beijing.Methods Peripheral blood lymphocytes from 38 healthy children and 46 asthmatic children were collected to detect the expression level of TRPV1 and TRPV2 mRNA by real-time quantitative PCR.Western blotting assay was used for quantification of total IgE levels and some specific IgE levels,including those of dust mites,pollen in spring and autumn,the hair of cockroaches,cats,dogs and mold.... |
| Keywords: | childhood asthma TRPV1 TRPV2 mRNA specific IgE |
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