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| 中国南海新型α-芋螺毒素Im1.6的克隆及合成 | |
| 引用本文： | 刘娜,刘珠果,孙婷,崔志芳,戴秋云. 中国南海新型α-芋螺毒素Im1.6的克隆及合成[J]. 军事医学科学院院刊, 2011, 35(8) |
| 作者姓名： | 刘娜 刘珠果 孙婷 崔志芳 戴秋云 |
| 作者单位： | 1. 山东科技大学化学与环境工程学院,山东青岛,266510;军事医学科学院生物工程研究所,北京,100071 2. 军事医学科学院生物工程研究所,北京,100071 3. 山东科技大学化学与环境工程学院,山东青岛,266510 |
| 基金项目： | 国家973计划项目(2010CB529802); 海洋863项目(2011AA090701); 国家自然科学基金项目(9071028) |
| 摘 要： | 目的从中国南海堂皇芋螺(Conus imperialis)中克隆出新的芋螺毒素序列并用化学方法合成该毒素。方法根据A超家族保守的信号肽序列设计引物,采用cDNA的3'端快速扩增方法(3'RACE),从芋螺毒腺管中克隆出新的毒素基因。固相法合成线性多肽,折叠形成目标产物,用两步氧化折叠法测定多肽的二硫键连接方式。结果发现一种新的α-芋螺毒素cDNA序列Im1.6,其编码的成熟肽序列为GCCDIPDCYNKNREQC-NH2,二硫键连接方式为C1-C3,C2-C4。结论 Im1.6是一种新的α4/7型芋螺毒素,其氨基酸序列与报道的α-芋螺毒素显著不同。 |
| 关 键 词： | 芋螺毒素类 4/7型 克隆 合成 二硫键骨架 |
Cloning and synthesis of a novel alpha conotoxin sequence Im1.6 from South China Sea |
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| LIU Na,LIU Zhu-guo,SUN Ting,CUI Zhi-fang,DAI Qiu-yun. Cloning and synthesis of a novel alpha conotoxin sequence Im1.6 from South China Sea[J]. Bulletin of the Academy of Military Medical Sciences, 2011, 35(8) | |
| Authors: | LIU Na LIU Zhu-guo SUN Ting CUI Zhi-fang DAI Qiu-yun |
| Affiliation: | LIU Na1,2,LIU Zhu-guo2,SUN Ting2,CUI Zhi-fang1,DAI Qiu-yun2 (1.College of Chemistry and Environmental Engineering,Shandong University of Science and Technology,Qingdao 266510,China,2.Institute of Biotechnology,Academy of Military Medical Sciences,Beijing 100071,China) |
| Abstract: | Objective To clone a new conotoxin from Conus imperialis derived from the South China Sea and synthesize the peptide.Methods Alpha-conotoxin was cloned in Conus venom by 3'RACE based on the conserved signal peptide sequence of A-super-family.The conotoxin was synthesized using solid-phase method and folded into the target product whose disulfide bridge connection was further determined by two-step oxidative folding.Results A novel conotoxin designated as Im1.6(GCCDIPDCYNKNREQC-NH2) was obtained,whose disulf... |
| Keywords: | conotoxins 4/7 subtype cloning synthesis disulfide bridge |
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