EphB4/EphrinB2在人脑星形细胞瘤中的表达及其病理学意义

目的：探讨EphrinB2与其受体EphB4、微血管密度（mierovessel density，MVD）和Ki-67在人脑星形细胞瘤中的表达及其病理学意义。方法：采用组织芯片技术和Maxvision^TM快捷免疫组织化学染色方法检测84例人脑星形细胞瘤和12例外伤脑组织中EphB4／EphrinB2、MVD和Ki-67的表达。结果：EphB4／EphrinB2、MVD、和Ki-67标记指数（Ki-67 labelling index．Ki-67LI）在人脑星形细胞瘤组和对照组之间的差异有统计学意义（P〈0．05）。EphB4、EphrinB2、MVD和Ki-67 LI与肿瘤级别呈正相关，MVD和Ki-67 LI与EphB4或EphrinB2蛋白表达正相关，有统计学意义（P〈0．05）。结论：EphB4和EphrinB2与人脑星形细胞瘤的分化程度和肿瘤血管生成密切相关，EphB4和EphrinB2的过度表达对肿瘤发展和促进肿瘤血管的生成起重要作用。利用EphB4／EphrinB2、MVD和Ki-67来判断人脑星形细胞瘤的恶性程度和病理特征，是一个较好的参考指标。

Expression of EphB4/EphrinB2 in human brain astrocytomas and its pathologic significance

Objective:To investigate the expressions of EphrinB2 and its receptor EphB4,microvessel density(MVD),and Ki-67 in human brain astrocytomas and explore their pathological significance.Methods: Expressions of EphB4/EphrinB2,MVD and Ki-67 were detected by tissue microarray and Maxvision shortcut immunohistochemical staining in 84 cases of human astrocytomas and 12 cases of traumatic brain tissues.Results: The difference was significant in expressions of EphB4/EphrinB2,MVD and Ki67 labeling index(LI) between human astrocytomas and controlled human traumatic brain tissues(P<0.05).Expression of EphB4/EphrinB2,MVD and Ki-67 LI had positive correlation with tumor grade.Ki-67 LI and MVD positively correlated with expressions of EphB4 or EphrinB2 protein(P<0.05).Conclusion:There is a close correlation between expression of EphB4/EphrinB2 and differentiation degree,tumor angiogenesis in human brain astrocytoma.The over-expressions of EphB4 and EphrinB2 play an important role in promoting tumor development and angiogenesis.EphB4/EphrinB2,MVD,and Ki-67 are fine reference parameters to estimate malignancy degree and pathologic features of human brain astrocytomas.