| 异功散通过抑制炎症反应促进慢性病贫血小鼠脾组织结构修复研究 |
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| 引用本文： | 季玉婷,郑秦,姜一陵,薛城,钟静娴,夏乐敏,张爱萍,石岭,戴程怡,罗梅宏. 异功散通过抑制炎症反应促进慢性病贫血小鼠脾组织结构修复研究[J]. 上海中医药杂志, 2019, 53(3): 67-71 |
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| 作者姓名： | 季玉婷 郑秦 姜一陵 薛城 钟静娴 夏乐敏 张爱萍 石岭 戴程怡 罗梅宏 |
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| 作者单位： | 上海市宝山区中西医结合医院上海中医药大学附属曙光医院宝山分院血液科 |
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| 基金项目： | 国家自然科学基金面上项目(81774271)；国家自然科学基金青年科学基金项目(81102569,81804021)；上海市自然科学基金项目(16ZR1425400)；上海市科学技术委员会引导类项目(17407931500)；上海市卫计委科研项目(20174Y0226) |
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| 摘 要： | 目的观察异功散通过抑制炎症反应,对慢性病贫血小鼠脾组织结构修复的影响。方法将60只8周龄雄性C57 BL/6小鼠随机分为空白对照组、模型组、异功散组,每组20只。除空白对照组外,其余各组复制慢性病贫血小鼠模型(第1天腹腔注射脂多糖,第7天腹腔注射酵母糖A)。注射酵母糖A后第5天,异功散组灌胃异功散水煎液(15.413 g·kg~(-1)·d~(-1)),空白对照组、模型组灌胃0.2 ml超纯水。各组分别于连续干预1周和2周后采集全血,自动细胞计数仪检测白细胞计数、中性粒细胞计数及血红蛋白水平;分离脾组织,HE染色观察脾组织结构,电镜观察小鼠脾脏线粒体情况。结果与空白对照组比较,模型组白细胞及中性粒细胞计数升高(P0.01),血红蛋白水平下降(P0.01);脾脏明显增大,脾脏指数增加(P0.01),脾组织线粒体正常结构消失。连续干预1周和2周后,与模型组比较,异功散组小鼠白细胞及中性粒细胞计数明显下降(P0.01),血红蛋白水平明显升高(P0.01);脾脏均明显缩小,脾脏指数降低(P0.01);电镜下观察到小鼠脾脏线粒体结构接近正常,受损线粒体数量减少。结论异功散可通过抑制炎症反应,减少炎症因子对脾组织结构的损伤,进而改善慢性病贫血小鼠的贫血状况。
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| 关 键 词： | 慢性病贫血 异功散 炎症反应 脾组织结构 小鼠 |
| 收稿时间： | 2018-10-11 |
Study on Yigong Powder promoting repair of spleen tissue structure by inhibiting inflammatory reactions in mice with anemia of chronic disease |
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| JI Yuting,ZHENG Qin,JIANG Yiling,XUE Cheng,ZHONG Jingxian,XIA Lemin,ZHANG Aiping,SHI Ling,DAI Chengyi and LUO Meihong. Study on Yigong Powder promoting repair of spleen tissue structure by inhibiting inflammatory reactions in mice with anemia of chronic disease[J]. Shanghai Journal of Traditional Chinese Medicine, 2019, 53(3): 67-71 |
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| Authors: | JI Yuting ZHENG Qin JIANG Yiling XUE Cheng ZHONG Jingxian XIA Lemin ZHANG Aiping SHI Ling DAI Chengyi LUO Meihong |
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| Affiliation: | Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China,Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China,Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China,Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China,Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China,Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China,Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China,Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China,Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China and Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China |
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| Abstract: | |
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| Keywords: | anemia of chronic disease Yigong Powder inflammatory reaction structure of spleen tissue mice |
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