| 白蒺藜抗光损伤小鼠光感受器细胞凋亡及保护视网膜功能的研究 |
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| 引用本文： | 徐静,吴晗晗,杜霄烨,张腾,陈瑜. 白蒺藜抗光损伤小鼠光感受器细胞凋亡及保护视网膜功能的研究[J]. 上海中医药杂志, 2019, 53(1): 76-79 |
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| 作者姓名： | 徐静 吴晗晗 杜霄烨 张腾 陈瑜 |
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| 作者单位： | 上海中医药大学附属岳阳中西医结合医院上海市中医药研究院中西医结合临床研究所 |
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| 基金项目： | 国家自然科学基金面上项目(81473732)；上海市教委曙光计划项目(13SG42)；上海市教委东方学者跟踪计划项目(沪教委人GZ2015011)；上海市教委东方学者计划项目(沪教委人201188) |
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| 摘 要： | 目的探讨白蒺藜对光损伤小鼠光感受器细胞凋亡及受损视网膜功能的影响。方法 BALB/c小鼠随机分为正常对照组、光损伤模型组、白蒺藜给药组。暗适应24 h后,白蒺藜给药组腹腔注射白蒺藜水煎液100μl(每20 g体质量100 mg生药),给药后30 min进行光照造模。造模采用白色荧光灯,光强度为10 000 lux,持续照射30 min。正常对照组和光损伤模型组同时腹腔注射等量0.9%NaCl溶液。(1)光照1 d、3 d后,摘取眼球制备冰冻切片,采用TUNEL法标记凋亡的光感受器细胞。(2)光照6 d后采用视网膜电流图(ERG)检测小鼠视网膜功能。结果 (1)TUNEL结果显示,正常对照组的外核层光感受器细胞核TUNEL染色为阴性。与正常对照组比较,光损伤后模型组小鼠的视网膜外核层TUNEL阳性光感受器在1 d开始显著增加,3 d达到高峰(P0.001);与光损伤模型组比较,白蒺藜给药组外核层仅见少量散在的TUNEL阳性光感受器细胞(P0.05)。(2)ERG结果显示,与正常对照组比较,光损伤模型组小鼠视网膜a波、b波振幅显著降低(P0.05),白蒺藜给药组a波、b波振幅显著高于模型组(P0.05)。结论白蒺藜能显著地减轻光损伤引起的光感受器细胞凋亡,对光损伤小鼠视网膜功能具有保护作用。
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| 关 键 词： | 白蒺藜 视网膜退行性病变 光感受器细胞凋亡 视网膜功能 小鼠 |
| 收稿时间： | 2018-10-16 |
Fructus Tribuli on suppressing photoreceptor apoptosis and preserving retinal function in bright light-exposed BALB/c mice |
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| XU Jing,WU Hanhan,DU Xiaoye,ZHANG Teng and CHEN Yu. Fructus Tribuli on suppressing photoreceptor apoptosis and preserving retinal function in bright light-exposed BALB/c mice[J]. Shanghai Journal of Traditional Chinese Medicine, 2019, 53(1): 76-79 |
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| Authors: | XU Jing WU Hanhan DU Xiaoye ZHANG Teng CHEN Yu |
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| Affiliation: | Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine; Clinical Research Department of Integrated Traditional Chinese and Western Medicine,Shanghai Institute of traditional Chinese Medicine,Shanghai 200437,China,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine; Clinical Research Department of Integrated Traditional Chinese and Western Medicine,Shanghai Institute of traditional Chinese Medicine,Shanghai 200437,China,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine; Clinical Research Department of Integrated Traditional Chinese and Western Medicine,Shanghai Institute of traditional Chinese Medicine,Shanghai 200437,China,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine; Clinical Research Department of Integrated Traditional Chinese and Western Medicine,Shanghai Institute of traditional Chinese Medicine,Shanghai 200437,China and Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine; Clinical Research Department of Integrated Traditional Chinese and Western Medicine,Shanghai Institute of traditional Chinese Medicine,Shanghai 200437,China |
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