| 基于代谢组学的菊粉改善脂质代谢紊乱的机制研究 |
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| 引用本文： | 段梦晨,王旭,胡佳亮,王新平,王源,冯怡. 基于代谢组学的菊粉改善脂质代谢紊乱的机制研究[J]. 上海中医药大学学报, 2019, 33(2): 80-85 |
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| 作者姓名： | 段梦晨 王旭 胡佳亮 王新平 王源 冯怡 |
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| 作者单位： | 上海中医药大学中药现代制剂技术教育部工程中心 |
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| 基金项目： | 国家自然科学基金青年基金资助项目(81803701)；上海市自然科学基金资助项目(16ZR1437200) |
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| 摘 要： | 目的:采用代谢组学技术研究菊粉对高脂饮食诱导的肥胖(DIO)小鼠脂质代谢紊乱的影响及其作用机制。方法:雄性C57BL/6J小鼠随机分为对照组、模型组和菊粉组,每组8只。对照组小鼠喂予普通饲料,模型组和菊粉组小鼠喂予高脂饲料诱导肥胖模型。造模8周后,菊粉组小鼠给予含8%菊粉的高脂饲料进行干预,对照组和模型组小鼠仍分别给予原饲料,继续喂养8周。试验期间每周记录小鼠体质量,末次给药后收集各组小鼠血清和粪便样本。全自动生化分析仪检测血清TC、TG、HDL-C和LDL-C的含量;气相色谱-飞行时间质谱(GC-TOF/MS)联用技术检测粪便样本,构建代谢轮廓谱,主成分分析(偏最小二乘法)比较组间代谢谱差异,筛选并鉴定差异性代谢物,分析代谢通路。结果:①菊粉干预第2周起,菊粉组小鼠体质量增长较模型组明显减缓;干预第6周,菊粉组小鼠平均体质量较模型组显著降低(P0.05),并持续至第8周。②干预8周后,与模型组相比,菊粉组小鼠血清TC、TG、HDL-C和LDL-C含量均显著下降(P0.05,P0.01)。③代谢组学主成分分析显示组间代谢谱差异明显。共鉴定11种潜在的差异性代谢物,与模型组相比,菊粉干预可升高果糖、麦芽糖、次黄嘌呤、油酸、肌酸和丁酸的含量,并降低棕榈酸、丙酮酸、色氨酸、尿嘧啶、3-羟基丁酸的含量,且通路分析显示丁酸代谢、淀粉和蔗糖代谢、不饱和脂肪酸的生物合成、脂肪酸生物合成等途径与菊粉改善DIO小鼠脂质代谢紊乱密切相关。结论:菊粉可有效改善高脂饮食诱导的肥胖小鼠脂质代谢紊乱,其机制可能与丁酸代谢、淀粉和蔗糖代谢、脂肪酸合成等通路有关。
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| 关 键 词： | 菊粉 脂质代谢紊乱 代谢组学 GC-TOF/MS 小鼠 |
| 收稿时间： | 2018-10-15 |
| 修稿时间： | 2018-11-14 |
Mechanism research on inulin improving lipid metabolism disorders based on metabolomics |
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| DUAN Mengchen,WANG Xu,HU Jialiang,WANG Xinping,WANG Yuan and FENG Yi. Mechanism research on inulin improving lipid metabolism disorders based on metabolomics[J]. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 2019, 33(2): 80-85 |
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| Authors: | DUAN Mengchen WANG Xu HU Jialiang WANG Xinping WANG Yuan FENG Yi |
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| Affiliation: | Engineering Center of Ministry of Education for Modern Preparation Technology of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Engineering Center of Ministry of Education for Modern Preparation Technology of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Engineering Center of Ministry of Education for Modern Preparation Technology of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Engineering Center of Ministry of Education for Modern Preparation Technology of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Engineering Center of Ministry of Education for Modern Preparation Technology of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China and Engineering Center of Ministry of Education for Modern Preparation Technology of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China |
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